AI Article Synopsis
- The study investigates how blocking the phospholipase C-gamma1 (PLC-gamma1) pathway affects the death of human glioma SWO cells when exposed to tumor necrosis factor-alpha (TNF-alpha).
- Researchers used U73122 to inhibit the PLC-gamma1 pathway and measured the effects on cell proliferation and apoptosis through various assays, including MTT and flow cytometry.
- Findings indicate that U73122 enhances the sensitivity of glioma cells to TNF-alpha-induced apoptosis, potentially by decreasing Bcl-2 expression, although blocking the pathway alone doesn’t cause apoptosis.
Article Abstract
Objective: To explore the role of phospholipase C-gamma1 (PLC-gamma1) in tumor necrosis factor-alpha (TNF-alpha)-induced apoptosis of human glioma SWO cells.
Methods: The PLC-gamma1 pathway was blocked by U73122 in SWO cells, and the inhibitory effect of TNF-alpha on SWO glioma cell proliferation with or without U73122 treatment was investigated by MTT assay. The cell apoptosis induced by TNF-alpha along or in combination with U73122 was detected by flow cytometry with PI staining. The expression of caspase-3 and Bcl-2 was detected by Western blotting.
Results And Conclusion: U73122 can sensitize SWO glioma cells to TNF-alpha-induced apoptosis. Blocking the PLC-gamma1 pathway may not induce apoptosis of SWO glioma cells, but can sensitize SWO glioma cells to small-dose TNF-alpha-induced apoptosis, the mechanism of which may involve down-regulation of bcl-2.
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