AI Article Synopsis
- The adhesion of cells to surfaces is significantly affected by the design of the surface pattern, which in this study was based on previously observed cell adhesion patterns.
- The researchers created a structured gold surface and used optical microscopy to analyze how focal adhesion kinase distributes on unstructured glass surfaces.
- Time-lapse studies showed that cells prefer to attach to the gold pads and shape themselves according to the pattern, with actin filaments aligning along the structures.
Article Abstract
Adhesion of adherent cells on structured surfaces is influenced by the surface pattern given. Here, we designed a structured gold relief surface based on cell adhesion patterns we had previously observed. We analysed the geometric parameters and the overall distribution of focal adhesion kinase in focal adhesions on unstructured glass surfaces using optical microscopy. The basic structural elements obtained from this analysis were arranged in regular clusters that resembled the shape of a polarised migratory cell. In time-lapse studies we observed that the cells adhere preferentially to the gold pads and adopt the shape of the clusters. Staining of the actin cytoskeleton revealed that the actin filaments are aligned to the gold pads of the elementary structure.
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| http://dx.doi.org/10.1016/j.ejcb.2005.08.007 | DOI Listing |
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