This review paper gives an overview of capillary electromigration methods used in the analysis of collagen. Analyses of the parent chains as well as of the bromcyane and collagenase fragments of collagens are presented. Methods include capillary zone electrophoresis, capillary gel electrophoresis, micellar electrokinetic chromatography as well as combinations of HPLC and capillary electrophoresis, and capillary electrophoresis with mass spectrometry.
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http://dx.doi.org/10.1016/j.jchromb.2006.02.043 | DOI Listing |
J Chromatogr A
January 2025
Department of Chemistry, Faculty of Science, POB 55 (A.I. Virtasen aukio 1), 00014 University of Helsinki, Helsinki, Finland. Electronic address:
This study was conducted to investigate possible differences in the interactions of some selected steroids based on their distribution coefficients with cholesterol- or ergosterol-rich liposomes. Structurally cholesterol and ergosterol have very close resemblance to each other and generally it is thought that they behave in a similar manner. In this work we will show that this is not the case.
View Article and Find Full Text PDFTalanta
January 2025
Translational Glycomics Research Group, Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Veszprem, Hungary; Horváth Csaba Memorial Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Faculty of Medicine, University of Debrecen, Hungary. Electronic address:
Sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) is a frequently used analytical technique in size-based separation of proteins, playing a vital role in the biopharmaceutical industry for the analysis and characterization of therapeutic proteins, employing both UV and fluorescent detection. Understanding the effect of the operational parameters using easily applicable in migratio fluorescent labeling is increasingly critical, especially because multicapillary electrophoresis systems with fluorescent detection have recently gained prominence in high-throughput biopolymer analysis. In this study, the effects of the three most important user-adjustable operational parameters (temperature, gel concentration, and electric field strength) were investigated on the electrophoretic mobility and resolution of SDS-protein complexes in the presence of propidium iodide in the gel-buffer system.
View Article and Find Full Text PDFAnal Chim Acta
February 2025
Department of Hygiene, Third Faculty of Medicine, Charles University, Ruská 87, 100 00, Prague 10, Czech Republic. Electronic address:
The review focuses on the design of detection cells, the use of microcontrollers for processing and evaluation of the detection signal, and the development of multi-detection systems for electromigration, liquid chromatography, flow-through and microfluidic techniques. A separate section is the introduction of modern 3D printing techniques and the use of new printing materials for the design of multidetection systems. In addition to traditional utilisation in separation techniques, new versions of contactless conductivity detectors are finding applications in FIA, SIA, portable and paper based analytical systems or as independent sensors.
View Article and Find Full Text PDFMolecules
January 2025
Institute of Environmental Engineering, Polish Academy of Sciences, M. Skłodowskiej-Curie 34, 41-819 Zabrze, Poland.
The cosmetics industry is one of the fastest-growing sectors worldwide. The dynamic evolution of this industry results in an increasingly diverse range of products containing various active ingredients. Ensuring the quality of these products is crucial for consumer safety, necessitating the use of advanced analytical methods and adherence to legal regulations.
View Article and Find Full Text PDFGels
December 2024
Horváth Csaba Memorial Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Faculty of Medicine, Doctoral School of Medicine, University of Debrecen, 4032 Debrecen, Hungary.
Hydrogels like agarose have long been used as sieving media for the electrophoresis-based analysis of biopolymers. During gelation, the individual agarose strands tend to form hydrogen-bond mediated double-helical structures, allowing thermal reversibility and adjustable pore sizes for molecular sieving applications. The addition of tetrahydroxyborate to the agarose matrix results in transitional chemical cross-linking, offering an additional pore size adjusting option.
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