AI Article Synopsis
- Talin plays a key role in integrin signaling by connecting to integrin cytoplasmic tails using its FERM domain made up of three subdomains (F1, F2, F3).
- The anti-talin monoclonal antibody TA205 disrupts actin stress fibers and focal adhesion in fibroblasts, indicating its impact on integrin activity.
- Research shows that TA205 inhibits the binding of integrin alphaIIb beta3 to the talin FERM domain and affects the interaction between talin’s head and rod regions, highlighting the significance of talin F1 in these processes.
Article Abstract
Talin mediates integrin signaling by binding to integrin cytoplasmic tails through its FERM domain which consists of F1, F2 and F3 subdomains. TA205, an anti-talin monoclonal antibody, disrupts actin stress fibers and focal adhesion when microinjected into fibroblasts. Here, we showed that TA205 caused an allosteric inhibition of integrin alphaIIb beta3 binding to the talin FERM domain and mapped the TA205 epitope to residues 131-150 in talin F1. Furthermore, binding of a talin rod fragment to talin head was partially inhibited by TA205. These findings suggest that talin F1 may be important in regulation of integrin binding and talin head-rod interaction.
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| http://dx.doi.org/10.1016/j.febslet.2006.02.077 | DOI Listing |
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