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Development of translationally active cell lysates from different filamentous fungi for application in cell-free protein synthesis.
Enzyme Microb Technol
January 2025
Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Universitätsplatz 1, Senftenberg 01968, Germany. Electronic address:
There is an enormous potential for cell-free protein synthesis (CFPS) systems based on filamentous fungi in view of their simple, fast and mostly inexpensive cultivation with high biomass space-time yields and in view of their catalytic capacity. In 12 of the 22 different filamentous fungi examined, in vitro translation of at least one of the two reporter proteins GFP and firefly luciferase was detected. The lysates showing translation of a reporter protein usually were able to synthesize a functional cell-free expressed unspecific peroxygenase (UPO) from the basidiomycete Cyclocybe (Agrocybe) aegerita.
View Article and Find Full Text PDFInvestigating the LiO-AlO-BO matrix: A promising approach for immobilizing radioactive waste.
Appl Radiat Isot
January 2025
Department of Physics, Faculty of Science, Universiti Teknologi Malaysia, 81300, Skudai, Johor, Malaysia.
Dealing with radioactive waste, particularly from various industrial processes, poses significant challenges. This paper explores the use of lithium aluminate borate (Li-Al-B) glass matrix as an alternative method for immobilizing radioactive waste, focusing specifically on waste generated in tin smelting industries, known as tin slag. The study primarily concentrates on transforming tin slag, a byproduct abundant in Natural Occurring Radioactive Material (NORM), into a stable and safe form for disposal.
View Article and Find Full Text PDFMuscle contributions to propelling the body upward differ between skipping and running.
J Biomech
January 2025
Walker Department of Mechanical Engineering, The University of Texas at Austin Austin TX USA.
Skipping represents a training alternative to running due to its lower knee contact forces and higher whole-body metabolic cost. The increased metabolic cost of skipping is associated with a higher vertical center-of-mass (COM) displacement during the support and flight phases of the skipping hop compared to running. However, skipping has lower muscle force impulses than running.
View Article and Find Full Text PDFLeveraging Artificial Intelligence to Develop a Decision-Support Tool for Visual Symptom Assessment of Grapevine Leafroll and Red Blotch Diseases.
Plant Dis
January 2025
University of California Davis, Cooperative Extension, Napa, California, United States;
The timely detection of viral pathogens in vineyards is a critical aspect of management. Diagnostic methods can be labor-intensive and may require specialized training or facilities. The emergence of artificial intelligence (AI) has the potential to provide innovative solutions for disease detection but requires a significant volume of high-quality data as input.
View Article and Find Full Text PDFHow a responsive reproduction factor is determinant in a prey-predator dynamics: A numerical and analytical study in a discrete-time model.
Chaos
January 2025
Physics Institute, University of São Paulo, 05508-090 São Paulo, SP, Brazil.
In this work, we investigate the dynamics of a discrete-time prey-predator model considering a prey reproductive response as a function of the predation risk, with the prey population growth factor governed by two parameters. The system can evolve toward scenarios of mutual or only of predators extinction, or species coexistence. We analytically show all different types of equilibrium points depending on the ranges of growth parameters.
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