AI Article Synopsis
- The study aimed to analyze how free calcium ions (Ca2+) are distributed in osteoclast-like cells grown on glass.
- Researchers used laser scanning confocal microscopy and a fluorescent probe to visualize and measure free Ca2+ levels within the cells.
- Results showed that free Ca2+ concentration was higher in the center of the cells compared to the edges, indicating that the uneven distribution of Ca2+ could play a role in how these cells regulate their functions.
Article Abstract
Objective: To study the spatial distribution of free Ca2+ in osteoclast-like cells cultured on glass.
Methods: To detect the free Ca2+ in osteoclast-like cells, the images were analyzed with image software, using the laser scanning confocal microscope and fluorescent probe.
Results: At 37 degrees C the free Ca2+ in osteoclast-like cells could be labelled effectively with 10 micromol/L Fluo-3/AM, the intensity of Ca2+ fluorescent signal in the central part was greater than that in the peripheral part and in the same section the signal was not distributed evenly.
Conclusion: The intensity of Ca2+ fluorescent signal is different among various organellae in osteoclast-like cell, which suggests the osteoclast-like cell modulate its own function through the spatial difference of free Ca2+ concentration.
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