AI Article Synopsis
- - The study aimed to explore how the expression of epidermal growth factor receptor (EGFR) changes in human periodontal ligament cells (hPDLC) during their mineralization process in a lab setting.
- - Researchers used methods like immunolocalization and RT-PCR to track changes in EGFR expression over time, finding that the presence of EGFR decreased significantly by the fourth week of the mineralization process.
- - The findings suggest that EGFR plays a negative regulatory role in the mineralization of hPDLC, indicating that as mineralization progresses, the expression of EGFR declines.
Article Abstract
Objective: To investigate the expression of epidermal growth factor receptor (EGFR) during the mineralization of human periodontal ligament cells (hPDLC) in vitro.
Methods: Studies using specific antibodies to immunolocalize EGFR in the mineral differentiating hPDLC were undertaken to investigate the different expression during the inducing process. In situ hybridization and RT-PCR technique were used to investigate the transcripts encoding the protein of EGFR.
Results: The results showed that immunocytochemical labeling gradually decreased following the elong of the induce time, downing to nearly negative at the 4th week and the signal of EGFR transcripts was weaker in the induced hPDLC than that in uninduced.
Conclusion: EGFR has a negative regulation function during the mineralization of hPDLC.
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