Quantitative measurement of DNA-DNA hybridization is a new technique for determination of unknown bacteria.It is more valuable how to identify strains which is rare difference on phenotypic characteristics.It is important to resolve bactericial taxionmy and identify new strain.Four strain of Streptiococcus are determined by hybridization of DNA.Some technique problems are discussed.Such as purification,precision of DNA and temperature as well as time of DNA hybridization and standard of judgment.
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