Background & Objective: NET-1 is a new tumor-related gene. So far, its expression in hepatocellular carcinoma (HCC) hasn't been reported. This study was to screen and compare the expression of NET-1 among fetal liver tissue, adult normal liver tissue, HCC and adjacent noncancerous tissues; explore its expression feature in HCC.
Methods: The expression of NET-1 mRNA in 3 specimens of fetal liver tissue, 4 specimens of adult liver tissue, 4 specimens of test HCC tissue, and 28 specimens of HCC and adjacent noncancerous tissues was detected by reverse transcription-polymerase chain reaction (RT-PCR). The expression level of NET-1 gene was analyzed by Four-Star image analysis software. NET-1 gene polyclonal antibody was generated by gene biological engineering, and its expression in human hepatic cancer cell line SMMC-7721 was observed under laser confocal microscope. The expression of NET-1 protein in the 28 specimens of HCC and adjacent noncancerous tissues was detected by immunohistochemistry.
Results: NET-1 mRNA was expressed in all of the 4 specimens of test HCC tissue, but didn't express in normal adult and fetal liver tissues; the positive rates of NET-1 mRNA were 85.71% (24/28) in both HCC and adjacent noncancerous tissues. The mRNA level of NET-1 was significantly higher in HCC than in adjacent noncancerous tissues (0.65 vs. 0.47, P<0.05). Observed under confocal microscope, NET-1 antibody was expressed in cytoplasm of SMMC-7721 cells. The positive rate of NET-1 protein was significantly higher in HCC than in adjacent noncancerous tissues (96.43% vs. 71.43%, P<0.05). In both HCC and adjacent noncancerous tissues, the positive rate of NET-1 mRNA was positively correlated to that of NET-1 protein (r=0.48, P<0.05; r=0.40, P<0.05).
Conclusion: NET-1 gene expression might be an early event in HCC development, and may have specific value in HCC diagnosis.
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