Acid-catalyzed hydrolysis was used as the mechanism to design a new type of environmentally sensitive fluorescence probe. A mild and selective periodate oxidation of the 2-amino alcohol of serine in the presence of a disulfide bond was developed to prepare dialdehyde peptides. Two identical fluorochrome hydrazide derivatives were then linked to the dialdehyde peptide forming an acid-labile hydrazone linkage. This self-quenched probe is weakly fluorescent at a physiological pH of 7.4 but shows more than 3-fold fluorescence enhancement at pH 4.5.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/bc050330e | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Stilbene-based fluorescent chemosensor for selective detection of cyanide ion in DMSO medium and its application in cell-imaging, fingerprint, and food sample analysis.
Spectrochim Acta A Mol Biomol Spectrosc
December 2024
Cancer Nanomedicine Lab, Department of Zoology, Periyar University, Salem, TN, India.
We designed a new cyanide sensing probe by one-step synthesis and evaluated it using UV-vis and fluorescent techniques. The active moiety of (Z)-3-(4-(methylthio) phenyl)-2-(4-nitrophenyl) acrylonitrile (NCS) demonstrated fluorescence. The probe NCS showed turn-off fluorescence in the presence of cyanide (CN¯), which has a higher selectivity and sensitivity than other anions.
View Article and Find Full Text PDFPyDNA-templated AgNPs coupled with poly (β‑cyclodextrin) enhanced fluorescence: A facile platform for signal amplification detection of biothiols in living cells and zebrafish.
Spectrochim Acta A Mol Biomol Spectrosc
December 2024
School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan 453003, PR China.
Accurate and sensitive fluorescence imaging of biothiols is essential for understanding the mechanism underlying some physiological and pathological events, as well as the prevention and diagnosis of diseases. However, low signal transduction efficiency and poor biocompatibility of fluorescence tags associated with current sensors hinder their potential utilizations. Herein, a smart biothiols sensitive vivo imaging platform on the basis of amplifying nanoprobe has been designed.
View Article and Find Full Text PDFTetraphenylethylene-indole as a novel fluorescent probe for selective and sensitive detection of human serum albumin (HSA) in biological matrices and monitoring of HSA purity and degradation.
Talanta
December 2024
Key Laboratory of Public Health Safety of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, School of Public Health, Hebei University, Baoding, 071002, PR China.
Human serum albumin (HSA) levels in serum and urine is a crucial biomarker for diagnosing liver and kidney diseases. HSA is used to treat various disorders in clinical practice and as an excipient in the production of vaccine or protein drug, ensuring its purity essential for patient safety. However, selective and sensitive detection of HSA remains challenging due to its structural similarity with bovine serum albumin (BSA) and the inherent complexity of biological matrices.
View Article and Find Full Text PDFPhotoelectrochemical aptasensing and fluorescence imaging co-joint detecting MCF-7 cells in whole blood via an inertial separation microfluidic chip.
Talanta
December 2024
Key Laboratory of Catalysis and Energy Materials Chemistry of Ministry of Education & Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, School of Chemistry and Materials Science, South-Central Minzu University, Wuhan, 430074, China. Electronic address:
The mortality rate of tumor is still very high till now. Circulating tumor cells (CTCs) are the major culprit of high cancer mortality. To improve survival rate of cancer patients, real-time monitoring and quantitative detection of CTCs are of indescribable value.
View Article and Find Full Text PDFLighting Up and Identifying Metal-Binding Proteins in Cells.
JACS Au
December 2024
Department of Chemistry and HKU-CAS Joint Laboratory of Metallomics for Health and Environment, The University of Hong Kong, Pokfulam Road, Hong Kong, SAR, P.R. China.
Metal ions, either essential or therapeutic, play critical roles in life processes or in the treatment of diseases. Proteins and enzymes are involved in metal homeostasis and the action of metallodrugs. Imaging and identifying these metal-binding proteins will facilitate the elucidation of metal-mediated life processes.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!