Objective: To prepare Microcystins-LR (MC-LR) conjugates with the carrier protein for developing a new immune assay for MC-LR.
Methods: MC-LR was coupled to BSA or KLH by "activated ester" and "water-soluble carbodimide". Measure the combine ratio of MC-LR with KLH or BSA by the UV scanning or the Bradford's method.
Results: The results are 9.1 and 9.4. New Zealand rabbits was immunized by MC-LR-KLH. The titer of the polyantibodies was over 25,600 measured by id ELISA.
Conclusion: MC-LR-KLH was proved the suitable immunogen against MC-LR.
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