AI Article Synopsis
- The centrosome is comprised of centrioles and surrounding material, and the study used specific antibodies to explore the localization of formiminotransferase cyclodeaminase (FTCD) in various cell types.
- FTCD was found to localize to the centrosome, particularly around the mother centriole, and this localization remained steady throughout the cell cycle, unaffected by Golgi fragmentation.
- The presence of FTCD at the centrosome suggests it may be linked to polyglutamylated microtubules, potentially contributing glutamate to the centrioles essential for their stability.
Article Abstract
In vertebrate cells, the centrosome consists of a pair of centrioles and surrounding pericentriolar material. Using anti-Golgi 58K protein antibodies that recognize formiminotransferase cyclodeaminase (FTCD), we investigated its localization to the centrosome in various cultured cells and human oviductal secretory cells by immunohistochemistry. In addition to the Golgi apparatus, FTCD was localized to the centrosome, more abundantly around the mother centriole. The centrosome localization of FTCD continued throughout the cell cycle and was not disrupted after Golgi fragmentation, which was induced by colcemid and brefeldin A. Centriole microtubules are polyglutamylated and stable against tubulin depolymerizing drugs. FTCD in the centrosome may be associated with polyglutamylated residues of centriole microtubules and may play a role in providing centrioles with glutamate produced by cyclodeaminase domains of FTCD.
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| http://dx.doi.org/10.1007/s00418-006-0166-5 | DOI Listing |
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