The Borrelia (B.) burgdorferi adhesin DbpA (decorin-binding protein A) is a valuable antigen for serodiagnosis of Lyme borreliosis and a promising candidate for a vaccine. To investigate the heterogeneity of DbpA, we aligned DNA sequences of 83 different dbpA genes (37 from the database, where the majority of sequences belong to B. burgdorferi sensu stricto and 46 were newly sequenced). Analysis of 25 sequences from the species B. burgdorferi s.s., 16 from B. afzelii, 40 from B. garinii, and two from the recently described human pathogenic genospecies A14S revealed five distinct DbpA groups. Group I comprises B. burgdorferi s.s. and group II B. afzelii. B. garinii is divided into groups III and IV, whereas A14S strains form group V. Formation of groups is mainly due to insertions of whole sequence sections. Comparison of dbpA sequences with ospC sequences from a subset of 59 strains revealed all kinds of cross-connections indicating processes of lateral gene transfer among strains. The extent of sequence identity within the dbpA genes decreases from the DNA (67%) to the amino acid (AA) level (44%) by about 23%, in contrast ospC sequence identities differed only by about 10%. This might be an indication that DbpA plays an important role in immune escape. Immunoblots using four recombinant DbpAs representing groups I-IV show that DbpA proteins are sensitive and specific antigens and complement one another in their reactivity. Part of the sera showed group-specific reactivity which could also be demonstrated with monoclonal antibodies.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ijmm.2006.01.006 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A simply designed quasi-ratiometric fluorescence probe for the visual and on-site detection of levofloxacin (LVF) residues in milk and fish sample.
Talanta
December 2024
College of Chemistry and Chemical Engineering, Henan Key Laboratory of Function-Oriented Porous Materials, Luoyang Normal University, Luoyang, 471934, China.
An europium metal organic framework (Eu-DBPA-Phen) was synthesized using 2,5-dibromoterephthalic acid (HDBPA) and 1-10-phenanthroline (Phen) as ligands. A straightforwardc quasi-ratiometric fluorescence probe was then developed for the detection of levofloxacin (LVF) by the simplistic combination of red-emitting Eu-DBPA-Phen and the inherent blue auto-fluorescence of the target. The probe exhibits the advantages of wide linear range (0.
View Article and Find Full Text PDFDNA-Binding Protein A Is Actively Secreted in a Calcium-and Inflammasome-Dependent Manner and Negatively Influences Tubular Cell Survival.
Cells
October 2024
Clinic of Nephrology, Hypertension, Diabetes and Endocrinology, Otto-von-Guericke University Magdeburg, 39120 Magdeburg, Germany.
DNA-binding protein A (DbpA) belongs to the Y-box family of cold shock domain (CSD) proteins that bind RNA/DNA and exert intracellular functions in cell stress, proliferation, and differentiation. Given the pattern of DbpA staining in inflammatory glomerular diseases, without adherence to cell boundaries, we hypothesized extracellular protein occurrence and specific functions. Lipopolysaccharide and ionomycin induce DbpA expression and secretion from melanoma and mesangial cells.
View Article and Find Full Text PDFDNA-binding protein-A promotes kidney ischemia/reperfusion injury and participates in mitochondrial function.
Kidney Int
August 2024
Clinic of Nephrology and Hypertension, Diabetes and Endocrinology, Otto-von-Guericke University Magdeburg, Magdeburg, Germany; Health Campus Immunology, Infectiology and Inflammation (GCI3), Otto-von-Guericke University Magdeburg, Magdeburg, Germany. Electronic address:
Article Synopsis
- DbpA (Ybx3) is part of the cold shock protein family and is involved in various cellular functions, with its role in acute kidney injury (specifically ischemia/reperfusion injury) being unclear.
- Research involving genetically modified mice showed that DbpA is not essential for kidney health but its deletion enhances mitochondrial performance and protects against IRI by reducing cell damage and immune response.
- The findings suggest that targeting DbpA could be beneficial in preventing kidney damage during surgeries like transplantation or cardiac procedures by improving mitochondrial function and reducing harmful cell processes.
Assessment of the hypothetical protein BB0616 in the murine infection of .
Infect Immun
June 2024
Department of Molecular Medicine, University of South Florida, Tampa, Florida, USA.
of , the Lyme disease pathogen, encodes a hypothetical protein of unknown function. In this study, we showed that BB0616 was not surface-exposed or associated with the membrane through localization analyses using proteinase K digestion and cell partitioning assays. The expression of was influenced by a reduced pH but not by growth phases, elevated temperatures, or carbon sources during cultivation.
View Article and Find Full Text PDFSmartphone-integrated paper-based sensing platform for the visualization and quantitative detection of pymetrozine.
Food Chem
May 2024
College of Chemistry and Chemical Engineering, Henan Key Laboratory of Function-Oriented Porous Materials, Luoyang Normal University, Luoyang 471934, China.
Pymetrozine (PYM) is an effective pyridine insecticide for controlling aphids, while its residues pose a serious threat to human health. Herein, a europium complex (Eu-DBPA, DBPA represents deprotonated 2,5-dibromoterephthalic acid ligand) probe was prepared for the detection of PYM via fluorescence quenching. The detection process has the advantages of short response time (2 min), wide linear range (0-4 and 4-45 mg/kg) and low detection limit (2.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!