AI Article Synopsis
- Swine brucellosis is caused by three Brucella suis biovars (1, 2, and 3), and current identification methods are often unreliable and labor-intensive.
- Researchers developed a multiplex PCR assay using a specific gene (omp2b) to categorize these biovars more effectively, revealing distinct amplification patterns for each biovar.
- The study found some field isolates labeled as biovars 2 or 3 still displayed patterns associated with biovar 1, indicating limitations in the new method's accuracy for typing B. suis.
Article Abstract
Swine brucellosis is caused by the biovars 1, 2 and 3 of Brucella suis the identification of which up to now relies on microbiological tests lacking adequate specificity together with time consuming and expensive molecular procedures. Based on sequence variation of the omp2b gene, we have developed a four primer set multiplex PCR assay that was tested for polymorphism analysis of B. suis biovars causing brucellosis in swine. The assay exploits the single nucleotide polymorphisms found in omp2b gene of B. suis reference biovars which are conserved in 43 B. suis field isolates from different geographic origins and hosts. Three specific amplification patterns (S1, S2 and S3) were obtained for reference strains of B. suis biovars 1, 2 and 3, respectively. However, some B. suis field isolates identified as biovars 2 or 3 according AMOS-PCR, PCR-RFLP of omp31 and omp2 genes and classical bacteriological methods, resulted also in S1 patterns, limiting the typing usefulness of the method.
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| http://dx.doi.org/10.1016/j.vetmic.2006.02.002 | DOI Listing |
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