Measurement of action spectra of light-activated processes.

J Biomed Opt

University of Queensland, Centre for Biophotonics and Laser Science, School of Physical Sciences, Saint Lucia, 4072 Queensland, Australia.

Published: June 2006

We report on a new experimental technique suitable for measurement of light-activated processes, such as fluorophore transport. The usefulness of this technique is derived from its capacity to decouple the imaging and activation processes, allowing fluorescent imaging of fluorophore transport at a convenient activation wavelength. We demonstrate the efficiency of this new technique in determination of the action spectrum of the light mediated transport of rhodamine 123 into the parasitic protozoan Giardia duodenalis.

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http://dx.doi.org/10.1117/1.2161172DOI Listing

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