AI Article Synopsis
- Junin virus strain Candid #1 is designed as a live attenuated vaccine for Argentine hemorrhagic fever and its L RNA nucleotide sequences are analyzed in comparison to its more virulent relatives.
- The study identified twelve unique point mutations in the L polypeptide of Candid #1 that may contribute to its less dangerous, or attenuated, nature.
- The Z ORF remained unchanged across all strains studied, indicating strong conservation in that region.
Article Abstract
Junin virus strain Candid #1 was developed as a live attenuated vaccine for Argentine haemorrhagic fever. In this paper, we report the nucleotide sequences of L RNA of Candid #1 and examine the relationship to its more virulent ancestors Junin virus XJ#44 and XJ 13 (prototype) and other closely and distantly related arenaviruses. Comparisons of the nucleotide and amino acid sequences of L and Z genes of Candid #1 and its progenitor strains revealed twelve point mutations in the L polypeptide that are unique to the vaccine strain. These changes could be provisionally associated with the attenuated phenotype. In contrast, Z ORF was completely conserved among all strains.
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| http://dx.doi.org/10.1007/s11262-005-5843-2 | DOI Listing |
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