Objective: To establish a method for whole genome amplification (WGA) based on (multiple displacement amplification MDA), and achieve DNA analysis of the human genome from low copy number (LCN).
Methods: DNA sample was used for WGA according to the REPLI-g kit protocol (QIAGEN, Germany). WGA product was used for DNA analysis according to the Applied Biosystems Profiler Plus kit protocol (ABI, USA).
Results: WGA product of DNA sample (10 pg) can be used for STR genotyping.
Conclusion: WGA technology could be helpful for LCN DNA analysis.
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