The Drosophila melanogaster hdc gene controls trachea branching, which starts during embryo development. Expression in imaginal disks and reproductive organs suggests additional functions for the hdc gene. The gene was demonstrated to have a maternal effect, which was denied previously. Analysis of cell proliferation in imaginal disks with hdc mutations showed that the gene does not possess tumor suppressor properties at the levels of mosaic cuticle clones of adults and transplanted imaginal disks. Transplanted imaginal disks homozygous, but not heterozygous, for an hdc mutation were found to affect oogenesis in the recipient females, implicating the hdc activity in exchanging signals between different organs. Amino acid sequence analysis of the Hdc protein revealed a region homologous to the human HRS proteins, which directly interact with the NF2 tumor suppressor on experimental evidence.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
A Simple Method to Analyze Context- and Tissue-Specific Cis-Regulatory Modulations of Homeotic (HOX) Genes Using ChIP.
Methods Mol Biol
January 2025
Department of Integrative Biology and Physiology, Medical School, Lillehei Heart Institute, University of Minnesota, Minneapolis, MN, USA.
Homeobox genes (HOX), the master regulators, deploy a unique set of target genes to coordinate and orchestrate the spatiotemporal development of an organism. HOX encoded transcriptional factors regulate the expression of target genes by binding to the specific sequences on the genome. Chromatin Immunoprecipitation (ChIP) and Chromatin Immunoprecipitation with Sequencing (ChIP-Seq) are widely used to map and understand specific gene locus and global regulatory regions on the genome.
View Article and Find Full Text PDFFasciclin 2 functions as an expression-level switch on EGFR to control organ shape and size in Drosophila.
PLoS One
December 2024
Instituto de Neurociencias CSIC-UMH, Universidad Miguel Hernandez, Sant Joan d'Alacant, Alicante, Spain.
Fasciclin 2 (Drosophila NCAM) is a homophilic Cell Adhesion Molecule expressed at moderate levels in the proliferating epithelial cells of imaginal discs, where it engages EGFR in a cell autonomous auto-stimulatory loop that promotes growth along larval development. In addition, Fasciclin 2 is expressed at high levels in the pre-differentiating cells of imaginal discs. Gain-of-function genetic analysis shows that Fasciclin 2 acts as a non-cell autonomous repressor of EGFR when high expression levels are induced during imaginal disc growth.
View Article and Find Full Text PDFOutstretched wing is controlled by intestinal enteroblasts-derived unpaired 2 cytokine signaling in Drosophila.
FASEB J
December 2024
Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, China.
Article Synopsis
- The study investigates how gut-derived signals affect wing development in fruit flies (Drosophila melanogaster), particularly focusing on the role of the Upd2 cytokine.
- It is found that mutations or overexpression of certain genes in intestinal cells can lead to a held-out wing phenotype and other health issues.
- The research highlights a gut-to-wing communication mechanism, suggesting that manipulating Upd2 expression can potentially correct developmental anomalies in wing formation.
Protocol for dissecting Drosophila pupae and visualizing RNA expression using hybridization chain reaction.
STAR Protoc
December 2024
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA; Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address:
Article Synopsis
- Visualizing RNA expression in the Drosophila epidermis during pupal development is difficult due to tissue fragility and impermeability at different stages.
- A new protocol is introduced for dissecting the tissue and detecting RNA in situ using hybridization chain reaction (HCR).
- This method allows researchers to study the changing patterns in gene expression throughout the various pupal stages.
Extramacrochaetae regulates Notch signaling in the eye through non-apoptotic caspase activity.
Elife
November 2024
Department of Genetics, Albert Einstein College of Medicine, Bronx, United States.
Many cell fate decisions are determined transcriptionally. Accordingly, some fate specification is prevented by Inhibitor of DNA-binding (Id) proteins that interfere with DNA binding by master regulatory transcription factors. We show that the Id protein Extra macrochaetae (Emc) also affects developmental decisions by regulating caspase activity.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!