Background: The authors evaluated the influence of cavity dimensions, insertion technique and adhesive system on microleakage of Class composite restorations.
Methods: The authors prepared cylindrical cavities with enamel margins of 3-millimeter diameter by 1-mm depth or 6-mm diameter by 2-mm depth on the labial surface of bovine incisors. They defined experimental groups (n = 15) according to cavity size, insertion technique (bulk or incremental) and adhesive system applied (Single Bond, 3M ESPE, St. Paul, Minn., or Excite, Ivoclar Vivadent, Amherst, N.Y.). They restored preparations with Tetric Ceram (Ivoclar Vivadent). After 36 hours' storage in distilled water at 37 C, specimens were submitted to microleakage using 50 percent silver nitrate as tracer. Teeth were sectioned twice and dye penetration on the axial wall was determined in millimeters.
Results: The authors analyzed the data using analysis of variance/Tukey test (a = .05). For large restorations, the use of Single Bond resulted in higher microleakage than Excite (1.56 +/- standard deviation [SD] 0.26 mm and 0.63 +/- SD 0.56 mm, respectively; P < .001), whereas for small restorations there was no statistical difference between adhesives (Excite: 0.47 +/- SD 0.28 mm; Single Bond: 0.46 +/- SD 0.28 mm).
Conclusion: The choice of adhesive system influenced microleakage only for large restorations. Incremental insertion of the composite did not affect dye penetration.
Clinical Implications: Microleakage of composite restorations cannot be predicted only on the basis of the restorations' dimensions. In large restorations, it also depends on the choice of adhesive system, whereas in small restorations, the adhesive used does not seem to be an influential factor. Incremental insertion did not seem to reduce restorations' microleakage.
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http://dx.doi.org/10.14219/jada.archive.2006.0145 | DOI Listing |
Clin Exp Med
January 2025
Department of Thoracic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
Introduction Recently, immune cells within the tumor microenvironment (TME) have become crucial in regulating cancer progression and treatment responses. The dynamic interactions between tumors and immune cells are emerging as a promising strategy to activate the host's immune system against various cancers. The development and progression of hepatocellular carcinoma (HCC) involve complex biological processes, with the role of the TME and tumor phenotypes still not fully understood.
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January 2025
Department of Vascular & Cardiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Purpose: Cardiomyocyte death is a major cytopathologic response in acute myocardial infarction (AMI) and involves complex inflammatory interactions. Although existing reports indicating that mixed lineage kinase domain-like protein (MLKL) is involved in macrophage necroptosis and inflammasome activation, the downstream mechanism of MLKL in necroptosis remain poorly characterized in AMI.
Methods: MLKL knockout mice (MLKL), RIPK3 knockout mice (RIPK3), and macrophage-specific MLKL conditional knockout mice (MLKL) were established.
Int J Biol Macromol
January 2025
Centre for Conservation and Utilization of Blue Green Algae, Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India. Electronic address:
Polysaccharides, found universally in all living-species, exhibit diverse biochemical structures and play crucial roles in microorganisms, animals, and plants to defend against pathogens, environmental stress and climate-changing. Microbial exopolysaccharides are essential for cell adhesion and stress resilience and using them has notable advantages over synthetic polysaccharides. Exopolysaccharides have versatile structures and physicochemical properties, used in food systems, therapeutics, cosmetics, agriculture, and polymer industries.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
National Engineering Research Center for Biotechnology, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, PR China; State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, PR China; Soochow University, Suzhou, Jiangsu 215123, PR China.
Pullulanase (PUL) plays a crucial role in breaking down α-1,6-glycosidic bonds in starch, a key process in starch processing and conversion. Based on PulB with high enzymatic activity, the expression of PUL in Bacillus subtilis was enhanced by plasmid screening, double promoter optimization, and signal peptide engineering. Furthermore, we innovatively employed a mussel foot protein to enhance the cell adhesion to carriers and utilized biofilm-based cell immobilization technology to optimize the fermentation process and stimulate biofilm formation.
View Article and Find Full Text PDFMar Pollut Bull
January 2025
Department of Materials Science and Engineering, Dalian Maritime University, Dalian 116026, PR China; Dalian Key Laboratory of Internal Combustion Engine Tribology and Reliability Engineering, Dalian 116026, PR China. Electronic address:
As a global challenge, marine biofouling is causing serious economic losses and adverse ecological impacts. In recent years, a variety of promising and environmentally friendly anti-fouling strategies have emerged, among which the excellent anti-fouling performance of bionic autocrine coatings has been recognized. However, bionic autocrine coatings still suffer from uncontrollable secretion behavior, poor mechanical stability, and poor abrasion resistance.
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