Twenty microsatellite markers selected from the chicken GENBANK were analyzed for polymorphism in two new Silkies laying breeds. Allele frequencies were tested by the auto scan on ABI 3100-Avant Genetic Analyzer. The results suggested that the optimization test of multiplex PCR was successful and 18 pairs of microsatellite DNA markers were polymorphic. The average number of alleles detected by a pair of primers was 7.444. The average value of PIC was 0.669. The average observation population heterzygosity of the 18 markers was 0.3962, and the average expected population heterzygosity of the 18 markers was 0.7301. The number of bands tested on the auto scan was higher than that based on band presence or absence on gel, thus it was more suitable for genetic population analysis.
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