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Quantitative analysis and characterization of biofunctionalized fluorescent silica particles. | LitMetric

AI Article Synopsis

  • A new method was developed to create biofunctionalized silica particles that can attach fluorescent dyes and oligonucleotide probes.
  • The particles showed a uniform distribution of amine groups after modification, ensuring effective coupling with the biomolecules.
  • The study also introduced a new way to quantify oligonucleotide loading using X-ray photoelectron spectroscopy, alongside a successful hybridization assay to showcase their use in DNA screening.

Article Abstract

A strategy for the production and subsequent characterization of biofunctionalized silica particles is presented. The particles were engineered to produce a bifunctional material capable of both (a) the attachment of fluorescent dyes for particle encoding and (b) the sequential modification of the surface of the particles to couple oligonucleotide probes. A combination of microscopic and analytical methods is implemented to demonstrate that modification of the particles with 3-aminopropyl trimethoxysilane results in an even distribution of amine groups across the particle surface. Evidence is provided to indicate that there are negligible interactions between the bound fluorescent dyes and the attached biomolecules. A unique approach was adopted to provide direct quantification of the oligonucleotide probe loading on the particle surface through X-ray photoelectron spectroscopy, a technique which may have a major impact for current researchers and users of bead-based technologies. A simple hybridization assay showing high sequence specificity is included to demonstrate the applicability of these particles to DNA screening.

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Source
http://dx.doi.org/10.1021/la052433rDOI Listing

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