We evaluated a two-step algorithm for detecting toxigenic Clostridium difficile: an enzyme immunoassay for glutamate dehydrogenase antigen (Ag-EIA) and then, for antigen-positive specimens, a concurrent cell culture cytotoxicity neutralization assay (CCNA). Antigen-negative results were > or = 99% predictive of CCNA negativity. Because the Ag-EIA reduced cell culture workload by approximately 75 to 80% and two-step testing was complete in < or = 3 days, we decided that this algorithm would be effective. Over 6 months, our laboratories' expenses were US dollar 143,000 less than if CCNA alone had been performed on all 5,887 specimens.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1393084 | PMC |
| http://dx.doi.org/10.1128/JCM.44.3.1145-1149.2006 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Commensal-pathogen dynamics structure disease outcomes during Clostridioides difficile colonization.
Cell Host Microbe
January 2025
The Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO, USA; Department of Pathology and Immunology, Division of Laboratory and Genomic Medicine, Washington University School of Medicine, St. Louis, MO, USA; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA; Department of Biomedical Engineering, Washington University in St Louis, St. Louis, MO, USA; Department of Pediatrics, Washington University School of Medicine, St. Louis, MO, USA. Electronic address:
Gastrointestinal colonization by Clostridioides difficile is common in healthcare settings and ranges in presentation from asymptomatic carriage to lethal C. difficile infection (CDI). We used a systems biology approach to investigate why patients colonized with C.
View Article and Find Full Text PDFGene Expression Dysregulation in Whole Blood of Patients with Infection.
Int J Mol Sci
November 2024
Department of Pharmacology and Therapeutics, Institute of Systems Molecular and Integrative Biology, University of Liverpool, Liverpool L69 3GL, UK.
() is a global threat and has significant implications for individuals and health care systems. Little is known about host molecular mechanisms and transcriptional changes in peripheral immune cells. This is the first gene expression study in whole blood from patients with infection.
View Article and Find Full Text PDFA quantitative PCR to detect non-toxigenic .
Microbiol Spectr
January 2025
Department of Pharmacy Practice and Translational Research, University of Houston College of Pharmacy, Houston, Texas, USA.
species lacking toxin genes (non-toxigenic or NTCD) may confer protection against CDI. However, current diagnostic tests detect either toxin proteins or toxin genes and cannot detect NTCD. This study developed a molecular testing method that uniquely identified NTCD and assessed its prevalence in a clinical cohort.
View Article and Find Full Text PDFComparison of the STANDARD M10 . , Xpert . , and BD MAX Cdiff assays as confirmatory tests in a two-step algorithm for diagnosing infection.
Microbiol Spectr
January 2025
Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea.
Current guidelines recommend a two-step algorithm rather than relying solely on a single test for diagnosing infection. This algorithm starts with enzyme immunoassay (EIA) for detecting glutamate dehydrogenase (GDH) and toxins A/B, followed by nucleic acid amplification test (NAAT) for GDH-positive but toxin-negative cases. This study compared the performance of three commercial NAATs: the STANDARD M10 , Xpert , and BD MAX Cdiff assays, utilized as confirmatory testing of the two-step algorithm.
View Article and Find Full Text PDFComplete genome sequences of toxigenic isolated from Australian feral horses.
Microbiol Resour Announc
December 2024
School of Biomedical Sciences, The University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands, Western Australia, Australia.
Recent increases in community-associated infections have highlighted the importance of monitoring toxigenic from animal and environmental sources. We provide the complete circularized genomes of two toxigenic strains isolated from feral horse faeces. Genome N64 (sequence type 964) consists of a single chromosome of 4,078,791 bp, while genome H251 (sequence type 963) comprises one chromosome (4,304,722 bp) and three plasmids (150,942 bp, 11,534 bp, and 9,074 bp).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!