Molecular diagnosis of Bordetella pertussis infection by evaluation of formalin-fixed tissue specimens.

J Clin Microbiol

Centers for Disease Control and Prevention, National Center for Infectious Diseases, Division of Viral and Rickettsial Disease, Infectious Disease Pathology Activity, 1600 Clifton Rd., NE, Mailstop G30, Atlanta, Georgia 30333, USA.

Published: March 2006

Formalin-fixed lung or trachea tissue specimens from four infants and one adolescent who died of respiratory illness were tested for Bordetella pertussis by conventional and real-time PCR assays. B. pertussis was confirmed in all cases. PCR can be an invaluable retrospective diagnostic tool for evaluating archival tissues from patients with suspected fatal pertussis.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1393166PMC
http://dx.doi.org/10.1128/JCM.44.3.1074-1076.2006DOI Listing

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