AI Article Synopsis
- The study focused on cultivating the entomopathogenic nematode Steinernema carpocapsae and its symbiotic bacterium, Xenorhabdus nematophila, in a specialized culture medium with ingredients like whey and yeast extract.
- A maximum concentration of 198,333 viable nematodes per milliliter was recorded after 24 days, with a significant portion of the population in the infective juvenile stage.
- The bioprocess dynamics were effectively modeled using a four-parameter Sigmoidal model, highlighting rates of nematode production and nutrient consumption, while also noting the bacterium's ability to hydrolyze lactose.
Article Abstract
The submerged culture of the entomopathogenic nematode Steinernema carpocapsae and its symbiotic bacterium, Xenorhabdus nematophila, was carried out in orbitally agitated bottles using a culture medium containing whey (in grams per litre: 500 whey, 20 yeast extract, 10 dried egg yolk-food grade, 3 sodium chloride, 37 corn oil-food grade). Maximum total viable nematode concentrations of 198,333ml(-1) were achieved within fermentations of 24 days with 64% of the nematode population within the infective juvenile stage (IJ) (126,666ml(-1)) at the end. The kinetics of the bioprocess was well modelled using the four-parameter Sigmoidal model and the corresponding maximum specific rates of nematode production (0.47 day(-1)), carbohydrates consumption (0.0008g(carbohydrates)g(nematodes)(-1)day(-1)) and nitrogen consumption (4.44g(nitrogen)g(nematodes)(-1)day(-1)) are first proposed. Besides, X. nematophila appears to have the capacity of lactose hydrolysis.
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| http://dx.doi.org/10.1016/j.jbiotec.2006.01.021 | DOI Listing |
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