Characterisation of recombinant Streptomyces spheroides NovW in vitro suggests that it is not a kinetically competent dual action dTDP-6-deoxy-d-xylo-4-hexulose 3,5-epimerase, but possesses only significant 3-epimerase activity.
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http://dx.doi.org/10.1039/b515763c | DOI Listing |
Mar Drugs
May 2021
National Key Laboratory of Biochemical Engineering, National Engineering Research Center for Biotechnology (Beijing), Key Laboratory of Biopharmaceutical Production & Formulation Engineering, PLA, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
It is known that bioactivities of chitooligosaccharide (COS) are closely related to the degree of polymerization (DP); therefore, it is essential to prepare COS with controllable DP, such as chitobiose showing high antioxidant and antihyperlipidemia activities. In this study, BLAST, sequence alignment and phylogenetic analysis of characterized glycoside hydrolase (GH) 46 -chitosanases revealed that a chitosanase Sn1-CSN from was different from others. Sn1-CSN was overexpressed in , purified and characterized in detail.
View Article and Find Full Text PDFJ Org Chem
August 2021
CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China.
A genome-directed discovery strategy to identify new tetrahydroisoquinolines (THIQs) was applied to deep-sea derived SCSIO 3406; 11 THIQs were found representing three THIQ classes. Known aclidinomycins A () and B () were isolated along with nine new compounds, aclidinomycins C-K (-). The structures were elucidated using extensive spectroscopic analyses and single-crystal X-ray diffraction methods.
View Article and Find Full Text PDFPLoS One
October 2021
CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.
Streptomyces niveus SCSIO 3406 was isolated from a sediment sample collected from South China Sea at a depth of 3536 m. Four new sesquiterpenoid naphthoquinones, marfuraquinocins A-D, and two new geranylated phenazines, i. e.
View Article and Find Full Text PDFCarbohydr Polym
May 2020
Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Comprehensive Laboratory Building, 350 Shushanhu Road, Hefei, Anhui 230031, People's Republic of China. Electronic address:
Prenyltransferase NovQ immobilized carboxymethyl cellulose magnetic nanoparticles (NCMNs) were successfully synthesized via a valuable approach integrated from nanocomposite preparation, and applied for the production of vitamin K using menadione hydroquinol and dimethylallyl diphosphate (DMAPP) as substrates. To investigate the interaction between nanoparticles and NovQ, we characterized the nanocomposite, and revealed that carboxymethyl cellulose (CMC) and FeO formed a core-shell structure to absorb NovQ in the reaction systems, resulting from the high affinity of immobilized materials. Meanwhile, NCMNs with excellent pH and temperature tolerance, enhanced prenylated activity, and improved stability were found.
View Article and Find Full Text PDFJ Recept Signal Transduct Res
February 2020
Department of Biotechnology and Microbiology, Kannur University, Kannur, Kerala, India.
Adenosine deaminase (ADA) is an enzyme present in purine metabolic pathway. Its inhibitors are considered to be potent drug lead compounds against inflammatory and malignant diseases. This study aimed to test ADA inhibitory activity of some Streptomyces secondary metabolites by using computational and methods.
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