Crystallization and preliminary crystallographic analysis of the (cytosine-5)-DNA methyltransferase NlaX from Neisseria lactamica.

Acta Crystallogr Sect F Struct Biol Cryst Commun

Max-Planck Unit for Structural Molecular Biology, Protein Dynamics Group, Hamburg 22607, Germany.

Published: September 2005

Crystals of the (cytosine-5)-DNA methyltransferase NlaX from Neisseria lactamica (molecular weight 36.5 kDa) have been grown at 291 K using 2.5 M NaCl as precipitant. The crystals diffract to 3.0 A resolution at 100 K. The crystals belong to space group P321, with unit-cell parameters a = 121.98, b = 121.98, c = 56.71 A. There is one molecule in the asymmetric unit and the solvent content is estimated to be 62.1% by volume.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1978117PMC
http://dx.doi.org/10.1107/S1744309105026709DOI Listing

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