[Construction and expression of fusion gene encoding Hyper-IL-6 in mammalian cells].

Aim: To construct and express fusion gene encoding Hyper-IL-6 in mammalian cells.

Methods: Using geneSOEing method, human sIL-6R cDNA was fused with IL-6 cDNA by a linker rich in glycine through PCR technique and cloned into pIRES(2)-EGFP. The constructed plasmid was transfected into mammalian cells. The expression of fusion gene encoding Hyper-IL-6 was detected by GFP and RT-PCR and immunocytochemistry.

Results: The recombinant plasmid pIRES-HIL-6 was successfully constructed and expressed in mammalian cells.

Conclusion: The success in construction and expression of human Hyper-IL-6 makes it possible to further study its biological functions.