AI Article Synopsis
- A Latex Agglutination Test (LAT) was created for the rapid detection of antibodies against H5 Avian Influenza Virus (AIV) in chickens using a recombinant hemagglutinin protein linked to latex beads.
- Optimization of conditions such as pH and ionic strength was done for effective sensitization.
- Testing on 126 serum samples showed the LAT had 87.03% sensitivity and 88.8% specificity, proving it a reliable method for quick antibody detection and epidemiological research on H5 AIV.
Article Abstract
A Latex Agglutination Test (LAT) was developed for quick detection of hemagglutinin serum antibodies of H5 Avian Influenza Virus (AIV) in chicken. Recombinant hemagglutinin protein of H5 AIV were obtained and purified, then HA protein were covalently linked to carboxylated polyethylene latex beads by EDAC. The sensitisation condition such as ionic strength of the diluting mixture pH, concentration, antigen, the times of linking were optimized. 126 fresh serum after vaccination were detected by this LAT and HI simultaneously, the result show that sensitivity for the LAT was 87.03%, the specificity was 88.8%, the coincidence rate of both methods was 87.30%. The result indicated that this LAT method can be used for quick detection of serum antibodies and epidemiological study of H5 AIV.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
All-positive HBsAg, HBsAb, HBeAg, HBeAb, and HBcAb in a patient with hepatitis B: A case report.
Medicine (Baltimore)
January 2025
Department of Clinical Immunology, Nanjing Kingmed Clinical Laboratory, Nanjing, Jiangsu, China.
Rationale: Mass vaccination, low cost of immunoglobulins, and new drugs led to the emergence of new, unusual patterns of hepatitis B serum markers. This study reported a rare case of hepatitis B with all 5 positive serum markers, including HBsAg, HBsAb, HBeAg, HBeAb, and HBcAb.
Patient Concerns: A 30-year-old female patient was admitted due to abnormal liver function.
Treatment of very high risk membranous nephropathy complicated by pulmonary embolism with glucocorticoids and rituximab: Case report.
Medicine (Baltimore)
January 2025
Division of Nephrology, Department of Medicine, National University Hospital, Singapore.
Rationale: We report the efficacy of combination prednisolone and intravenous (IV) rituximab as an immunosuppressive regimen for a young male presenting with extensive venous thromboembolism including a submassive pulmonary embolism secondary to life-threatening nephrotic syndrome from very high risk anti-phospholipase-A2 receptor (PLA2R) positive membranous nephropathy. Initial treatment was with mechanical thrombectomy and anticoagulation. Thereafter, oral prednisolone was initiated to induce remission, during a period of uninterrupted anticoagulation.
View Article and Find Full Text PDFWhat is Rheumatoid factor-from screening to personalized management.
Rheumatology (Oxford)
January 2025
The First Department of Internal Medicine, University of Occupational and Environmental Health, Japan.
The rheumatoid factor (RF) is a representative autoantibody against the crystallizable fragment (Fc) of denatured immunoglobulin (Ig) G that are primarily detected in patients with rheumatoid arthritis (RA). Although five types of tumor necrosis factor (TNF) inhibitors can be used to treat RA, no guidelines are available for selecting the appropriate inhibitor for treatment. High serum RF levels are associated with high disease activity, progressive joint destruction, life prognosis associated with organ damage, decreased treatment responsiveness to TNF inhibitors and other drugs, and low treatment retention rates.
View Article and Find Full Text PDFEvaluation of an F Protein-Based Recombinant Protein for Immunization Against Respiratory Syncytial Virus.
Viral Immunol
January 2025
Department of Microbiology, Basic Science Center, Autonomous University of Aguascalientes, Aguascalientes, Mexico.
Respiratory syncytial virus (RSV) is one of the most important etiologies of acute respiratory infections that cause bronchiolitis in children under 5 years of age. Treatments are expensive, no vaccine is available, and this is an important cause of hospitalization. Costimulatory molecules have been reported to be good inducers of antiviral type 1 immune response.
View Article and Find Full Text PDFA Recombinant Antibody Against ALK2 Promotes Tissue Iron Redistribution and Contributes to Anemia Resolution in a Mouse Model of Anemia of Inflammation.
Am J Hematol
January 2025
Keros Therapeutics, Lexington, Massachusetts, USA.
Patients with chronic inflammation are burdened with anemia of inflammation (AI), where inflammatory cytokines inhibit erythropoiesis, impede erythropoietin production, and limit iron availability by inducing the iron regulator hepcidin. High hepcidin hinders iron absorption and recycling, thereby worsening the impaired erythropoiesis by restricting iron availability. AI management is important as anemia impacts quality of life and potentially affects morbidity and mortality.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!