A direct rapid immunohistochemical test (dRIT) was evaluated under field and laboratory conditions to detect rabies virus antigen in frozen and glycerol-preserved field brain samples from northwestern Tanzania. Compared to the direct fluorescent antibody test, the traditional standard in rabies diagnosis, the dRIT was 100% sensitive and specific.
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http://dx.doi.org/10.3201/eid1202.050812 | DOI Listing |
J Vasc Surg Venous Lymphat Disord
January 2025
Department of Vascular Surgery, Chenzhou First People's Hospital and the First Affiliated, Hospital of Xiangnan University, Chenzhou, Hunan Province 423000, China. Electronic address:
Objective: This study sought to investigate the changes in plasma D-dimer levels during catheter-directed thrombolysis (CDT) in patients with acute lower extremity deep venous thrombosis (DVT), analyze imaging results, and assess their clinical implications.
Methods: We retrospectively analyzed 62 patients diagnosed with acute lower extremity DVT who underwent CDT between March 2019 and December 2022. Plasma D-dimer levels were measured before CDT, at regular intervals after CDT, and at the end of CDT.
Talanta
January 2025
Department of Materials and Environmental Technology, Tallinn University of Technology, Ehitajate tee 5, 19086, Tallinn, Estonia. Electronic address:
Ampicillin (AMP) ranks third among the top ten most frequently sold antibiotic combinations globally, raising concerns due to its extensive use. Improper disposal practices in agriculture, aquaculture, and healthcare have led to environmental contamination of water sources with elevated AMP levels. Current methods for detecting such contamination are costly, require sophisticated equipment, and depend on skilled personnel and unstable natural receptors.
View Article and Find Full Text PDFMicrobiol Spectr
January 2025
Faculty of Chemistry, Biotechnology and Food Science, NMBU - Norwegian University of Life Sciences, Ås, Norway.
Unlabelled: a natural inhabitant of the human body, is a promising candidate vehicle for vaccine delivery. An obstacle in developing bacterial delivery vehicles is generating a production strain that lacks antibiotic resistance genes and contains minimal foreign DNA. To deal with this obstacle, we have constructed a finetuned, inducible two-plasmid CRISPR/Cas9-system for chromosomal gene insertion in .
View Article and Find Full Text PDFJ Am Chem Soc
January 2025
National Center for International Research on Green Optoelectronics, Guangdong Provincial Key Laboratory of Optical Information Materials and Technology, Institute of Electronic Paper Displays, South China Academy of Advanced Optoelectronics, South China Normal University, Guangzhou 510006, China.
Ice melting, a common yet complex phenomenon, remains incompletely understood. While theoretical studies suggest that preexisting defects in ice generate "off-lattice" water molecules, triggering bulk ice melting, direct experimental evidence of their form has been lacking as the transparent and transient nature of ice poses significant challenges for observation with current techniques. Here, we introduce an ice-melting-induced lyophilization (IMIL) technique that employs graphene-based nanoprobes to replicate and track liquid evolution within melting bulk ice.
View Article and Find Full Text PDFIn this research, we report a simple fluorescent probe designed to detect thallium(iii) ions (Tl) in artificial urine samples. The Tl signaling probe (TP-1) was readily prepared from 2-acetyl-6-methoxynaphthalene and hydrazine. In a pH 4.
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