AI Article Synopsis
- The study aimed to understand how calcium ions (Ca2+) affect gene expression during electroporation-mediated gene transfer in mice.
- Results showed that adding just 10 mmol/L Ca2+ significantly decreased transgene expression to less than 5% of normal levels, with no other ions having the same effect.
- The inhibitory impact of Ca2+ appears to be linked to changes in the cell membrane after electroporation, affecting how well the gene transfer works.
Article Abstract
Aim: To investigate the specific effects of Ca2+ on transgene expression during electroporation-mediated gene transfer in mice.
Methods: Skeletal muscle and skin were subjected to in vivo electroporation with a luciferase reporter plasmid, with or without Ca2+ and various other ions.
Results: For in vivo electroporation, the presence of just 10 mmol/L Ca2+ in the DNA solution drastically reduced the resulting transgene expression, to less than 5% of control values. Only Ca2+, not other ions, caused inhibition, and the effect was not tissue specific. More surprisingly, even when Ca2+ ions were delivered by electroporation before or after DNA administration, similar effects were still observed.
Conclusion: The inhibitory effect of Ca2+ on in vivo gene transfer by electroporation is specific, ie, the inhibitory effect may be related to the cell membrane properties after electroporation and the subsequent resealing event.
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| http://dx.doi.org/10.1111/j.1745-7254.2006.00280.x | DOI Listing |
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