Background: Abscission is the regulated dropping of plant organs, such as leaves or flower petals. This process involves a break down of the cell wall between layers of cells in the abscission zone, causing the organ to become detached. The model plant Arabidopsis thaliana undergoes floral organ abscission. Various experimental methods have been used to study Arabidopsis floral organ abscission, including measuring the petal breakstrength, or the amount of force required to pull a petal from the receptacle. Petal breakstrength provides a quantitative insight into the physical integrity of the petal abscission zone.
Results: We developed a petal breakstrength meter that allows rapid data acquisition on a personal computer. We present the design of the device and show its utility in measuring Arabidopsis petal breakstrength for abscission studies.
Conclusion: This petal breakstrength meter should enable researchers to perform the petal breakstrength assay as a routine part of the characterization of environmental and genetic factors affecting abscission.
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http://dx.doi.org/10.1186/1746-4811-2-2 | DOI Listing |
Methods Mol Biol
January 2019
Department of Molecular Biosciences, University of Oslo, Oslo, Norway.
Petal breakstrength (pBS) is a method to study floral organ abscission by quantitating the force required to pull a petal from the receptacle. However, it is only well established in some labs and used in a subset of abscission studies. Here, we describe the mechanism and operation of the pBS meter, as well as detailed measurement and further data analysis.
View Article and Find Full Text PDFMethods Mol Biol
February 2018
Department of Horticulture, University of Wisconsin, 486 Horticulture Bldg., 1575 Linden Drive, Madison, WI, 53706, USA.
Abscission is the developmentally controlled loss of plant organs, providing diverse functions such as shedding senescent or diseased leaves, dispersing seeds, or dropping ripened fruit. This process is defined by the hydrolytic breakdown of the middle lamella in the abscission zone, allowing for organ detachment. While the model plant Arabidopsis thaliana does not undergo leaf abscission, it does have a predictable progression of floral organ abscission.
View Article and Find Full Text PDFJ Exp Bot
December 2013
Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway.
Floral organ shedding is a cell separation event preceded by cell-wall loosening and generally accompanied by cell expansion. Mutations in NEVERSHED (NEV) or INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) block floral organ abscission in Arabidopsis thaliana. NEV encodes an ADP-ribosylation factor GTPase-activating protein, and cells of nev mutant flowers display membrane-trafficking defects.
View Article and Find Full Text PDFPlant Physiol
May 2013
Sutton Bonington Campus, School of Biosciences, Plant and Crop Sciences Division, University of Nottingham, Loughborough, Leicestershire, UK.
A number of novel strategies were employed to examine the role of indoleacetic acid (IAA) in regulating floral organ abscission in Arabidopsis (Arabidopsis thaliana). Analysis of auxin influx facilitator expression in β-glucuronidase reporter plants revealed that AUXIN RESISTANT1, LIKE AUX1, and LAX3 were specifically up-regulated at the site of floral organ shedding. Flowers from mutants where individual family members were down-regulated exhibited a reduction in the force necessary to bring about petal separation; however, the effect was not additive in double or quadruple mutants.
View Article and Find Full Text PDFDevelopment
April 2008
Department of Botany, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada.
The Arabidopsis BLADE-ON-PETIOLE 1 (BOP1) and BOP2 genes encode redundant transcription factors that promote morphological asymmetry during leaf and floral development. Loss-of-function bop1 bop2 mutants display a range of developmental defects, including a loss of floral organ abscission. Abscission occurs along specialised cell files, called abscission zones (AZs) that develop at the junction between the leaving organ and main plant body.
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