A potentially broad-range PCR system for gltA gene amplification has been designed. A nearly full (992 bp) sequence of the gltA gene was amplified in two steps. DNA was extracted from organs (spleen and liver) of four mostly spread species of rodents: Apodemus agrarius and Apodemus peninsulae mice; Clethrionomys rufocanus and Microtus fortis voles. All amplicons have been sequenced. The incidence of Bartonella species in rodents was 73%, 60%, 60%, and 83% for the above-mentioned rodents, respectively. Several genotypes have been identified, including two gltA genotypes close to B. taylorii, four gltA genotypes close to B. grahamii, and one genotype of a potentially new species. Several Bartonella species were found in both mice species, although each vole species harbored a single species of Bartonella. High incidence of Bartonella infection was found in all studied species of small rodents in the Russian Far East. Several amplified genotypes probably belong to local strains of B. taylorii and B. grahamii, and one genotype represents a possible new species.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1196/annals.1355.049 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Transcriptional induction of the IMD signaling pathway and associated antibacterial activity in the digestive tract of cat fleas (Ctenocephalides felis).
Parasit Vectors
December 2024
Department of Biology, Georgia Southern University, 4324 Old Register Rd., Statesboro, GA, 30460, USA.
Background: Fleas are insect vectors that transmit several Gram-negative bacterial pathogens acquired by ingesting infected vertebrate blood. To combat foodborne illness, insect midgut epithelial cells are armed with efficient microbial recognition and control systems, such as the immune deficiency (IMD) pathway that regulates the expression of antimicrobial peptides (AMPs). However, despite their medical and veterinary importance, relatively little is known about the IMD signaling pathway and production of AMPs in the digestive tract of cat fleas (Ctenocephalides felis).
View Article and Find Full Text PDFDetection and phylogenetic analysis of blood-associated pathogens from spleen samples of wild raccoons (Procyon lotor) in Germany.
Sci Rep
December 2024
Department of Zoology, Musée National d'Histoire Naturelle, Luxembourg, Luxembourg.
Raccoons (Procyon lotor) originated in North America and have been introduced to Europe. Due to their close contact with human settlements, they are important reservoirs for zoonotic pathogens, such as Baylisascaris procyonis. The relevance and prevalence of vector-borne pathogens have not yet been fully elucidated.
View Article and Find Full Text PDFEpidemiological characteristics and genetic diversity of Bartonella species from rodents in Guangxi Zhuang autonomous region, Southwestern China.
Acta Trop
December 2024
Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, Henan province, China; Key Laboratory of Veterinary Biotechnology of Henan Province, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, Henan, China. Electronic address:
Bartonella spp. are gram-negative bacteria recognized as zoonotic pathogens of wide spectrum mammals. Rodents are recognized as a natural reservoir of pathogens, and many Bartonella species transmitted by various blood-sucking arthropods have been detected in various rodents populations.
View Article and Find Full Text PDFYield of clinical metagenomics: insights from real-world practice for tissue infections.
EBioMedicine
December 2024
Department of Laboratory Medicine, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, PR China; Zhejiang Key Laboratory of Clinical in Vitro Diagnostic Techniques, Hangzhou, 310003, PR China; Institute of Laboratory Medicine, Zhejiang University, Hangzhou, 310003, PR China. Electronic address:
Background: While metagenomic next-generation sequencing (mNGS) has been acknowledged as a valuable diagnostic tool for infections, its clinical validity and impact on patient management when using fresh tissue samples remains uncertain.
Methods: We conducted a retrospective cross-sectional study involving patients who underwent tissue mNGS at a tertiary hospital in China from February 2021 to February 2024, aiming to assess its ability to detect plausible pathogens and its clinical validity and impact.
Findings: A total of 520 mNGS results from 508 patients were analysed, detecting plausible pathogens in 302 (58.
Editorial: Insights in glomerular disease.
Front Nephrol
December 2024
Division of Nephrology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, United States.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!