In the present study, isolation of anaerobic bacteria from 24 different eco-niches was carried out. A total number of 300 bacterial isolates, including 230 obligate and 70 facultative anaerobes were obtained using anaerobic techniques. All the isolates were initially screened for succinic acid production by Fluorescein test and TLC method. During screening, 10 isolates found to produce succinic acid were further examined by HPLC and then finally confirmed for succinic acid by LC-MS analysis. Amongst 10 isolates, isolate SAP, a facultative anaerobe isolated from buffalo rumen fluid, showed maximum yield of 2.1 g/l of succinic acid from 10 g of glucose in 24 hr under anaerobic condition. This isolate was identified as Klebsiella pneumoniae strain SAP by 16S rDNA sequence and signature sequence analysis. Mouse lethality test for the strain SAP showed LD50 value of 3.3 x 10(8) CFU/ml, which shows non-virulent nature of the strain. This strain may become a candidate strain for succinic acid production because of its osmotolerant nature and higher succinate:acetate ratio.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
The molecular pathways leading to GABA and lactic acid accumulation in florets of organic broccoli rabe ( subsp. ) stored as fresh or as minimally processed product.
Hortic Res
January 2025
Institute for Biological Systems, National Research Council (CNR), Via Salaria Km 29,300, 00015 Monterotondo, Rome, Italy.
In the context of organic farming, the introduction of a local product to wider markets and an evaluation of storage effects, metabolic and transcriptomic variations in two broccoli rabe genotypes from production cycles of two different years were studied by comparing florets of stored fresh (SF) and packaged (P) for 4 days with those harvested fresh from the field (H). Twenty-five hydrosoluble compounds, including amino acids, carbohydrates, and organic acids, were quantified by untargeted nuclear magnetic resonance (NMR). Principal component analysis produced a neat separation among the three commodity statuses with P being the most divergent and SF closer to H.
View Article and Find Full Text PDFA study on the qualitative analysis of lotus seedpod oligomeric procyanidins during digestion, absorption and colonic fermentation based on UPLC-Q-Exactive/MS.
Food Chem
January 2025
Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Key Laboratory of Fermentation Engineering (Ministry of Education), National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan, Hubei 430068, China. Electronic address:
Polyphenols have potent antioxidant properties, but are easily degraded in the gastrointestinal tract, greatly limiting their application as dietary supplements. Therefore, the composition changes of lotus seedpod oligomeric procyanidins (LSOPC) in the gastrointestinal digestion, colonic fermentation and their absorption in Caco-2 cell monolayer were studied. The extracted LSOPC were identified using UPLC-Q-Exactive/MS, and a total of 47 compounds were identified.
View Article and Find Full Text PDFMicrobial succinate promotes the response to metformin by upregulating secretory immunoglobulin a in intestinal immunity.
Gut Microbes
December 2025
Beijing Institute of Clinical Pharmacy, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
Metformin is the first-line pharmacotherapy for type 2 diabetes mellitus; however, many patients respond poorly to this drug in clinical practice. The potential involvement of microbiota-mediated intestinal immunity and related signals in metformin responsiveness has not been previously investigated. In this study, we successfully constructed a humanized mouse model by fecal transplantation of the gut microbiota from clinical metformin-treated - responders and non-responders, and reproduced the difference in clinical phenotypes of responsiveness to metformin.
View Article and Find Full Text PDFFree radical synthesis of succinic anhydride grafted poly(lactic acid) porous templates for sustained drug delivery in the buffer media.
Int J Biol Macromol
January 2025
Department of Chemistry, Faculty of Arts and Science, Turkey Suleyman Demirel University, Faculty of Arts and Science, 32260 Isparta, Turkey.
Poly(lactic) (PLA) is a biodegradable material obtained from renewable resources and is recognized as a safe biopolymer by the Food and Drug Administration. PLA expresses excellent mechanical and moldability attributes nonetheless poor elasticity/functionality limits its widespread utilization. One approach to compensate for this is chemical surface modification through free radical grafting with small organic molecules like maleic anhydride (MA).
View Article and Find Full Text PDFIntermetallic RNiSi (R = Ca, La, and Y) Catalysts with Electron-Rich Ni Sites for Continuous Flow Selective Hydrogenation of Maleic Anhydride.
ACS Appl Mater Interfaces
January 2025
State Key Laboratory of Fine Chemicals, Laboratory of Advanced Materials and Catalytic Engineering, School of Chemical Engineering, Dalian University of Technology, Dalian 116024, China.
The industrial advancement of downstream products resulting from the directed hydrogenation of maleic anhydride is hindered by the limitations related to the activity and stability of catalysts. The development of nonprecious metal intermetallic compounds, in which active sites are adjustable in the local structures and electronic properties embedded within a distinct framework, holds immense potential in enhancing catalytic efficacy and stability. Herein, we report that nickel-based silicides catalysts, RNiSi (R = Ca, La, and Y), afford high efficiency in the selective hydrogenation of maleic anhydride.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!