[The molecule mechanism of soybean isoflavaones antagonizing bone loss induced by postmenopausal osteoporosis].

Objective: To observe the effect of the Soybean Isoflavaones on the mRNA expression of Osteoprotegerin (OPG), Osteoclast Differentiation Factor (ODF) and Macrophage Colony stimulating Factor (M-CSF) in bone tissue of ovariectomized rat, and to investigate the possible molecule mechanism of Soybean Isoflavaones antagonizing bone lose induced by postmenopausal osteoporosis.

Methods: Thirty healthy adult SD rats were randomly divided into 3 groups: sham-operated group, ovariectomized group and Soybean Isoflavaones treated group. All rats were ovariectomized except those in sham-operated group. Bone density of the 3-6th lumbar vertebrae were detected after 12 weeks. Total RNA were extracted from femur bone and the mRNA expression of OPG, ODF and M-CSF were examined by real time PCR.

Results: The data showed that Soybean Isoflavaones increased the bone density of the ovariectomized rat lumbar vertebrae and up-regulated the expression of OPG,whereas down-regulated the expression of M-CSF and the ratio of ODF:OPG.

Conclusion: The molecule mechanism of Soybean Isoflavaones antagonizing bone loss induced by postmenopausal osteoporosis is tightly correlated with the regulation of expression of OPG,M-CSF and the ratio of ODF:OPG.