AI Article Synopsis
- The study aimed to investigate the production of bacteriocin by Shigella flexneri strains and its connection to dysenteric diarrhea, as well as the genetic factors involved.
- Out of 116 strains analyzed, 49 were found to produce bacteriocin that was effective against certain Escherichia coli and non-bacteriocinogenic Sh. flexneri strains, with specific DNA used to transform E. coli cells.
- The research concluded that only Sh. flexneri strains from dysenteric cases produced bacteriocin, which is linked to a 3 kb plasmid responsible for this antibacterial substance.
Article Abstract
Aims: To determine the production of bacteriocin by Shigella flexneri strains, to relate their production to the presence of dysenteric diarrhoea and to asses the genetic determination of the bacteriocin.
Methods And Results: One hundred and sixteen strains of Sh. flexneri were isolated from patients with diarrhoea and 49 of them produced bacteriocin active against several Escherichia coli and abacteriocinogenic Sh. flexneri strains. The extrachromosomal DNA isolated from bacteriocinogenic Sh. flexneri strains were used as a substrate to transform E. coli HB-101 cells by means of electroporation.
Conclusions: Only the Sh. flexneri strains isolated from dysenteric diarrhoea produced bacteriocin. It was demonstrated that a plasmid of approx. 3 kb was responsible for the genetic determination of these anti-bacterial substances.
Significance And Impact Of The Study: A 3-kb plasmid that harboured information for the production of bacteriocin by Sh. flexneri strains was described. The production of this bacteriocin may be related to dysenteric diarrhoea produced by these bacterial strains.
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| http://dx.doi.org/10.1111/j.1472-765X.2005.01829.x | DOI Listing |
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