We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage phiC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1363781 | PMC |
| http://dx.doi.org/10.1093/nar/gnj019 | DOI Listing |
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