The transport factor p40 is thought to assist Rab9 in mediating late-endosome-to-TGN vesicular transport. p40 was recently identified as an associated protein of the PtdIns 5-P/PtdIns 3,5-P2-producing kinase PIKFyve. Moreover, p40 recovery in membrane fractions appeared totally dependent on the presence of an intact enzymatic activity of PIKFyve, implying a mechanism of p40 membrane association dependent on membrane PtdIns 5-P and/or PtdIns 3,5-P2. Here we have evaluated plausible interaction of recombinant p40 with the PIKFyve products PtdIns 5-P and PtdIns 3,5-P2 by a synthetic-liposome binding assay.
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