Structural changes of cell wall mannans of Candida guilliermondii IFO 10279 strain cells cultured at high temperature.

The morphology, structure, and antigenicity of the cells and the cell wall mannans of the Candida guilliermondii IFO 10279 strain cultivated at 33 and 34 degrees C for 48 h in yeast extract-added Sabouraud liquid medium (YSLM) were compared with those cultivated at 27 degrees C and 33 degrees C and then at 27 degrees C (33-27 degrees C). This strain showed little growth at higher than 35 degrees C. The density of the yeast formed cells decreased, with dry weights of about 50% at 33 and 34 degrees C, and only the cells at 34 degrees C revealed a failure of cytokinesis. The structure of the mannans revealed by (1)H-NMR analysis that the mannans obtained at both 33 and 34 degrees C had drastically decreased two consecutive beta-1,2-linked mannopyranose units at the nonreducing terminal of the alpha-linked oligosaccharides and increased one beta-1,2-linked mannopyranose unit at the nonreducing terminal attached to the alpha-1,3-linked mannose unit and the non-reducing terminal alpha-1,3- and alpha-1,2-linked mannopyranose units. The enzyme-linked immunosorbent assay (ELISA) showed that the mannans obtained at 33 and 34 degrees C had decreased reactivity against the factor serum 9 and increased its reactivity against the factor serum 4, in the commercially available factor serum kit "Candida Check".