Objectives: To investigate possible protective effects of vitamin E and Ham's F-10 medium (HF-10) on lipid peroxidation, apoptosis, motility, and vitality of spermatozoa.
Methods: Normozoospermic semen samples were obtained from 35 volunteers. Normal saline solution, HF-10 only, or HF-10 with vitamin E were added to split semen samples (control, group 1, and group 2, respectively). Sperm motility and vitality were evaluated at the end of 1, 2, and 24 hours. Superoxide dismutase, catalase, and malondialdehyde levels were assessed at the end of the first hour. Flow cytometric DNA analysis was performed at the end of 24 hours.
Results: Superoxide dismutase, sperm motility, and vitality were not different among the groups. The catalase values decreased in group 1, but not in group 2. Malondialdehyde values in supernatants decreased in group 2 and apoptosis of spermatozoa decreased in groups 1 and 2.
Conclusions: Our data suggest that vitamin E and HF-10 protect against the reactive oxygen species-mediated damage on spermatozoa.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.urology.2005.08.022 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Impact of NADPH Oxidase 5 Activity and Reactive Oxygen Species on Capacitated Human Sperm.
J Reprod Infertil
January 2024
Department of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Background: Progesterone (P4) activates sperm calcium channels (CatSper), allowing calcium to enter the cell, which activates NADPH Oxidase-5 (NOX5) and produces reactive oxygen species (ROS). While calcium and ROS are essential for sperm capacitation, the role of NOX5 in capacitated sperm is unclear. This study investigated NOX5 activity in capacitated human sperm.
View Article and Find Full Text PDFAn islet maturation media to improve the development of young porcine islets during in vitro culture.
Islets
May 2020
Department of Surgery, University of California, Irvine , Orange, CA, USA.
Background: The use of pancreata from pre-weaned piglets has the potential to serve as an unlimited alternative source of islets for clinical xenotransplantation. As pre-weaned porcine islets (PPIs) are immature and require prolonged culture, we developed an islet maturation media (IMM) and evaluated its effect on improving the quantity and quality of PPIs over 14 days of culture.
Methods: PPIs were isolated from the pancreata of pre-weaned Yorkshire piglets (8-15 days old).
Inhibition of CatSper and Hv1 Channels and NOX5 Enzyme Affect Progesterone-Induced Increase of Intracellular Calcium Concentration and ROS Generation in Human Sperm.
Iran J Med Sci
March 2019
Department of Immunology, Infertility Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Background: Normal sperm function depends on appropriate intracellular calcium (Ca ) and reactive oxygen species (ROS) levels. Calcium activates NADPH oxidase-5 (NOX5) that leads to ROS generation. The calcium channel of sperm (CatSper) is activated by progesterone and intracellular alkalization.
View Article and Find Full Text PDFEffect of CatSper and Hv1 Channel Inhibition on Progesterone Stimulated Human Sperm.
J Reprod Infertil
January 2018
Department of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Background: Intracellular calcium and proton concentrations are important factors for activating human sperm. Calcium ion (Ca) enters sperm through voltage-dependent calcium channel of sperm (CatSper). Proton was extruded from sperm through voltage-gated proton channel (Hv1).
View Article and Find Full Text PDFCharacterization and cryopreservation of Amur leopard cats (Prionailurus bengalensis euptilurus) semen collected by urethral catheterization.
Theriogenology
October 2018
College of Veterinary Medicine, Chungbuk National University, Cheongju, 28644, Republic of Korea. Electronic address:
The Amur leopard cat (Prionailurus bengalensis euptilurus) is a globally endangered species, and there is thus an urgent need to increase its population. The objectives of this study were to: (1) confirm the utility of urethral catheterization (UC) for semen collection from Amur leopard cats; (2) investigate proper dilution media for fresh semen; and (3) investigate the effectiveness of sperm cryopreservation, including examining the effect of glycerol concentration. Six adult males in captivity (mean weight 5.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!