AI Article Synopsis
- Yeast studies have identified genes for protein secretion, but the processes in more complex organisms like Drosophila are less understood.
- A genome-wide RNA interference screen in Drosophila cells was used to find genes necessary for basic protein secretion and how they impact Golgi organization.
- Depletion of identified gene classes resulted in different effects on Golgi membranes, such as redistribution, fragmentation, and aggregation, while some genes showed no significant change.
Article Abstract
Yeast genetics and in vitro biochemical analysis have identified numerous genes involved in protein secretion. As compared with yeast, however, the metazoan secretory pathway is more complex and many mechanisms that regulate organization of the Golgi apparatus remain poorly characterized. We performed a genome-wide RNA-mediated interference screen in a Drosophila cell line to identify genes required for constitutive protein secretion. We then classified the genes on the basis of the effect of their depletion on organization of the Golgi membranes. Here we show that depletion of class A genes redistributes Golgi membranes into the endoplasmic reticulum, depletion of class B genes leads to Golgi fragmentation, depletion of class C genes leads to aggregation of Golgi membranes, and depletion of class D genes causes no obvious change. Of the 20 new gene products characterized so far, several localize to the Golgi membranes and the endoplasmic reticulum.
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| http://dx.doi.org/10.1038/nature04377 | DOI Listing |
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