The experiments were made in laboratory to analyze the characteristics and principle of benzene's biodegradation using the microorganism (G-, Flavobacterium) taken from Daqing oil fields. Results show that the maximal concentration of benzene, which microorganisms could endure is between 8.8 mg x L(-1) and 17.6 mg x L(-1); microorganisms were inhibited as benzene's concentration was beyond 17.6 mg x L(-1). Trends of benzene's concentration in and out of microorganism's cell are almost same; the biodegradation could be achieved efficiently as the pH range of 6.5 - 7.0 and benzene initial concentration range of 7.04 - 13.2 mg x L(-1). Change of - lgP (P is the distributive ratio of benzene in membrane and water) could be illustrated the trends of toxicity and degradation of benzene in and out of microorganism's cell; the biodegradation rate varied simultaneously with the change of P as initial concentration of benzene was beyond 8.8 mg x L(-1).
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