AI Article Synopsis
- Melanocytes and neuroblasts both convert L-tyrosine into products via different pathways: melanogenesis for melanocytes and catecholamines for neuroblasts.
- A study on the glioblastoma cell line ADF showed that these cells express tyrosinase and L-tyrosine hydroxylase, and are capable of synthesizing melanosomes.
- The research also found that L-tyrosine can reduce PPARalpha expression in glioblastoma cells and can trigger apoptosis in both glioblastoma and neuroblastoma cells.
Article Abstract
Melanocytes and neuroblasts share the property of transforming L-tyrosine through two distinct metabolic pathways leading to melanogenesis and catecholamine synthesis, respectively. While tyrosinase (TYR) activity has been shown to be expressed by neuroblastoma it remains to be established as to whether also glioblastomas cells are endowed with this property. We have addressed this issue using the human continuous glioblastoma cell line ADF. We demonstrated that these cells possess tyrosinase as well as L-tyrosine hydroxylase (TH) activity and synthesize melanosomes. Because the two pathways are potentially cyto-genotoxic due to production of quinones, semiquinones, and reactive oxygen species (ROS), we have also investigated the expression of the peroxisomal proliferators activated receptor alpha (PPARalpha) and nuclear factor-kB (NFkB) transcription factor as well the effect of L-tyrosine concentration on cell survival. We report that L-tyrosine down-regulates PPARalpha expression in ADF cells but not neuroblastoma and that this aminoacid and phenylthiourea (PTU) induces apoptosis in glioblastoma and neuroblastoma.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jcp.20603 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Repeated fasting events sensitize enhancers, transcription factor activity and gene expression to support augmented ketogenesis.
Nucleic Acids Res
January 2025
Institute of Biochemistry, Food Science and Nutrition. The Robert H. Smith Faculty of Agriculture, Food and Environment. The Hebrew University of Jerusalem. 229 Herzl Street, Rehovot 7610001, Israel.
Mammals withstand frequent and prolonged fasting periods due to hepatic production of glucose and ketone bodies. Because the fasting response is transcriptionally regulated, we asked whether enhancer dynamics impose a transcriptional program during recurrent fasting and whether this generates effects distinct from a single fasting bout. We found that mice undergoing alternate-day fasting (ADF) respond profoundly differently to a following fasting bout compared to mice first experiencing fasting.
View Article and Find Full Text PDFA feasibility study using quantitative and interpretable histological analyses of celiac disease for automated cell type and tissue area classification.
Sci Rep
December 2024
Eli Lilly and Company, Indianapolis, IN, USA.
Histological assessment is essential for the diagnosis and management of celiac disease. Current scoring systems, including modified Marsh (Marsh-Oberhuber) score, lack inter-pathologist agreement. To address this unmet need, we aimed to develop a fully automated, quantitative approach for histology characterisation of celiac disease.
View Article and Find Full Text PDFAcute and Chronic Resistance Training, Acute Endurance Exercise, nor Physiologically Plausible Lactate In Vitro Affect Skeletal Muscle Lactylation.
Int J Mol Sci
November 2024
School of Kinesiology, Auburn University, Auburn, AL 36849, USA.
Article Synopsis
- The study analyzed how resistance exercise and endurance cycling affect protein lactylation and acetylation in skeletal muscles using biopsies from college-aged participants.
- Results showed that while acute and chronic resistance training reduced cytoplasmic protein acetylation, they did not significantly impact protein lactylation.
- In vitro tests with sodium lactate treatments also found no significant changes in protein lactylation or acetylation, suggesting that exercise primarily influences protein acetylation rather than lactylation.
Erratum to "Novel co-initiators of polymerization: Cytotoxicity profile and modulation of inflammatory mediators in human dental pulp stem cells" [Dent Mater 40/10 (2024) 1692-1696].
Dent Mater
January 2025
Dental Research Division, Paulista University, Rua Doutor Bacelar, 1212, Zip Code: 04026-002 Sao Paulo, Brazil.
Assessment of 10-MDP and GPDM monomers on viability and inflammatory response in human dental pulp stem cells.
Dent Mater
January 2025
Dental Research Division, Paulista University, Rua Doutor Bacelar, 1212, Zip Code: 04026-002 Sao Paulo, Brazil. Electronic address:
Objectives: to assess the cytotoxicity of the following functional monomers used in dental adhesives: 10-Methacryloyloxydecyl dihydrogen phosphate (10-MDP) and glycerol phosphate dimethacrylate (GPDM), and their effect on cytokine release from human dental pulp stem cells (hDPSCs).
Methods: The hDPSCs cells were isolated from the dental pulp of extracted human third molars. The functional monomers, 10-MDP and GPDM, were diluted in dimethyl sulfoxide (DMSO) at concentrations ranging from 1 to 4 mM.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!