AI Article Synopsis
- The study identified glycosidic compounds in nutmeg, including p-cymene-7-ol, eugenol, methoxyeugenol, and alpha-terpineol, by measuring their concentrations using a TLC densitometer.
- The major glycoside found was p-cymene-7-ol rutinoside (3.15 mg/100 g), and the effects of gamma radiation were assessed, revealing that radiation significantly decreases glycoside content, with alpha-terpineol glucoside being the most sensitive.
- A new method was suggested for quickly isolating and quantifying aroma glycosides by extracting them into n-butanol, which simplifies the process compared to previous
Article Abstract
Glycosidically bound volatile compounds of nutmeg were identified as glyco-conjugates of p-cymene-7-ol, eugenol, methoxyeugenol and alpha-terpineol. Using phenyl-beta-glucoside as external standard the contents of these glycosidic precursors were estimated based on the measurement of TLC spot density on a densitometer. p-Cymene-7-ol rutinoside was the major aroma glycoside (3.15 mg/100 g), followed by glucosides of methoxyeugenol (0.61 mg/100 g), eugenol (0.50 mg/100 g) and alpha-terpineol (0.51 mg/100 g). A dose-dependent breakdown of these glycosidic precursors was observed during gamma-radiation processing. Among the four glycosides, alpha-terpineol glucoside was the most sensitive to radiation while p-cymene-7-ol rutinoside was the least sensitive. A reduction in the content of total glycosides by almost 50% was noted at a dose of 5kGy. Partitioning of aroma glycoside into n-butanol from aqueous extracts was found to result in rapid isolation of aroma glycosides, avoiding time consuming pre-purification on Amberlite XAD-2 column. A routine method based on extraction into n-butanol and subsequent quantification of post-irradiation changes in aroma glycosides on a TLC plate using a densitometer is proposed.
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| http://dx.doi.org/10.1016/j.chroma.2006.01.009 | DOI Listing |
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