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Gold Nanoprobes for Detection of a Crucial EGFR Deletion for Early Diagnosis of Non-Small-Cell Lung Cancer.
Biosensors (Basel)
March 2024
LAQV/REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua Campo Alegre, 687, 4169-007 Porto, Portugal.
Gold nanoparticles (AuNPs) exhibit improved optical and spectral properties compared to bulk materials, making them suitable for the detection of DNA, RNA, antigens, and antibodies. Here, we describe a simple, selective, and rapid non-cross linking detection assay, using approx. 35 nm spherical Au nanoprobes, for a common mutation occurring in exon 19 of the epidermal growth factor receptor (EGFR), associated with non-small-cell lung cancer cells.
View Article and Find Full Text PDFA simple strategy based on combinatorial gold nanoparticle sizes for enhanced sensitivity in colorimetric bioanalysis.
J Mater Chem B
May 2023
Analytical & Testing Center, Sichuan University, Chengdu, Sichuan, 610064, China.
Taking advantage of salt-induced aggregation, gold nanoparticles (AuNPs) have been intensively employed in non-cross-linking strategies (NCLs) for colorimetric bioanalysis. Such a classical method is popular due to its simplicity and cost-effectiveness, but it still suffers from poor sensitivity in analytical practice. To address this issue, we simply mixed the four sizes of non-functional AuNPs (10 nm/20 nm/30 nm/40 nm) to establish a highly sensitive combinatorial system in a non-cross-linking strategy (cNCL).
View Article and Find Full Text PDFDNA-Programmed Bimodal 2D Assembly of Differently Sized Nanoparticles via Folding of Precursory Circular Chains.
Langmuir
May 2020
Department of Advanced Materials Science, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwano-ha, Kashiwa, Chiba 277-8561, Japan.
Gold nanoparticle (AuNP) assemblies in two-dimensions (2D) exhibit collective physical/chemical properties that are useful for various devices. However, technical issues still impede the efficient ordering of differently sized AuNPs on solid supports while avoiding phase separation. This paper describes a method to construct binary 2D assemblies by folding precursory circular chains composed of small and large AuNPs.
View Article and Find Full Text PDFRNA Quantification Using Noble Metal Nanoprobes: Simultaneous Identification of Several Different mRNA Targets Using Color Multiplexing and Application to Chronic Myeloid Leukemia Diagnostics.
Methods Mol Biol
January 2021
Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, UCIBIO, Universidade Nova de Lisboa, Caparica, Portugal.
Nanotechnology provides new tools for gene expression analysis that allow for sensitive and specific characterization of prognostic signatures related to cancer. Cancer is a complex disease where multiple gene loci contribute to the phenotype. The ability to simultaneously monitor differential expression originating from each locus allows for a more accurate indication into the degree of cancerous activity than either locus alone.
View Article and Find Full Text PDFColorimetric-based detection of using gold nanoparticles.
IET Nanobiotechnol
February 2020
Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
(uu) is one of the most common agents of urogenital infections and is associated with complications such as infertility, spontaneous abortion and other sexually transmitted diseases. Here, a DNA sensor based on oligonucleotide target-specific gold nanoparticles (AuNPs) was developed, in which the dispersed and aggregated states of oligonucleotide-functionalised AuNPs were optimised for the colorimetric detection of a polymerase chain reaction (PCR) amplicon of DNA. A non-cross-linking approach utilising a single Au-nanoprobe specific of the urease gene was utilised and the effect of a PCR product concentration gradient evaluated.
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