Distinct roles for the essential MYST family HAT Esa1p in transcriptional silencing.

Mol Biol Cell

Division of Biological Sciences, UCSD Cancer Center and Center for Molecular Genetics, University of California-San Diego, La Jolla, CA 92093, USA.

Published: April 2006

Among acetyltransferases, the MYST family enzyme Esa1p is distinguished for its essential function and contribution to transcriptional activation and DNA double-stranded break repair. Here we report that Esa1p also plays a key role in silencing RNA polymerase II (Pol II)-transcribed genes at telomeres and within the ribosomal DNA (rDNA) of the nucleolus. These effects are mediated through Esa1p's HAT activity and correlate with changes within the nucleolus. Esa1p is enriched within the rDNA, as is the NAD-dependent protein deacetylase Sir2p, and the acetylation levels of key Esa1p histone targets are reduced in the rDNA in esa1 mutants. Although mutants of both ESA1 and SIR2 have enhanced rates of rDNA recombination, esa1 effects are more modest yet result in distinct structural changes of rDNA chromatin. Surprisingly, increased expression of ESA1 can bypass the requirement for Sir2p in rDNA silencing, suggesting that these two enzymes with seemingly opposing activities both contribute to achieve optimal nucleolar chromatin structure and function.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1415314PMC
http://dx.doi.org/10.1091/mbc.e05-07-0613DOI Listing

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