AI Article Synopsis
- The study aimed to determine how basophil allergen sensitivity (CD-sens) could aid in monitoring anti-IgE treatment for patients allergic to timothy grass pollen.
- Significant correlations were found between CD-sens and other allergen sensitivity measures, such as skin prick test results and serum IgE concentrations, indicating its reliability.
- The researchers concluded that CD-sens is a more effective method for assessing a patient's allergen sensitivity and treatment monitoring than the traditional CD-max method, which may produce misleading results.
Article Abstract
Background: Monitoring of the allergen sensitivity of a patient is most important for optimal patient care and a basic prerequisite for immunomodulating treatment. The objective of this study was to investigate how basophil allergen sensitivity can be applied in the monitoring of anti-immunoglobulin E (IgE) treatment.
Methods: Basophils from timothy grass pollen allergic patients were, by flow cytometry, analysed for allergen threshold sensitivity (CD-sens) by measuring CD63 up-regulation on CD203c-identified basophils. The results were compared with maximal percentage CD63 up-regulation at one allergen dose (CD-max), skin prick test end-point allergen titration, (SPT-sens), nasal provocation titration tests (nasal provocation titre) and serum IgE and IgE antibody concentrations.
Results: There was a significant correlation (r = 0.50, P = 0.01) between CD-sens and SPT-sens, CD-sens and the IgE antibody concentration in percentage of 'total IgE' (relative IgE antibody concentration) (r = 0.72, P < 0.001) as well as between CD-sens and nasal provocation titre (r = 0.54, P < 0.05) but, in contrast, CD-max did not correlate with any of the sensitization parameters, i.e. SPT-sens, nasal provocation titre, absolute and relative IgE antibody concentration or CD-sens. CD-sens could be used to monitor omalizumab treatment efficacy while, based on CD-max, four of seven symptom-free patients on omalizumab would have been classified as having ongoing allergy.
Conclusions: CD-sens seems to be very useful for the determination of a patient's allergen sensitivity and should be evaluated for the measurement and monitoring of anti-IgE treatment efficacy. CD-max, the conventional approach to basophil allergen challenge, which mirrors cell reactivity, gives incorrect information.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1398-9995.2006.00987.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Transcriptomic Profiles of the Nasal Mucosa Following Birch Pollen Provocation Differ Between Birch Pollen-Allergic and Non-Allergic Individuals.
Allergy
December 2024
Department of Dermatology, University Hospital St. Poelten, Karl Landsteiner University of Health Sciences, St. Poelten, Austria.
Article Synopsis
- Birch pollen (BP) exposure leads to significant changes in gene expression and cytokine levels in allergic individuals compared to non-allergic individuals, indicating distinct biological responses.
- RNA sequencing identified 160 genes activated in allergic participants, with specific pathways related to inflammation and immune response being enriched in their nasal secretions.
- The findings suggest that BP allergy triggers unique signaling mechanisms in predisposed individuals, providing insights for future research into allergy treatments.
Repetitive Calf-Raise Pulse Provocative Test for Popliteal Artery Entrapment Syndrome.
Cureus
November 2024
Trauma and Orthopaedics, Wythenshawe Hospital, Manchester University NHS Foundation Trust, Manchester, GBR.
Popliteal artery entrapment syndrome (PAES) is a rare cause of exertional leg pain in young adults, which is caused by compression of the popliteal artery by the surrounding muscular structure. Due to significant overlap in symptoms with other conditions, limitations of diagnostic imaging, and lack of awareness, PAES is frequently misdiagnosed, resulting in late complications and poor prognosis. Clinical assessment is crucial in making the initial diagnosis and referring for relevant investigations for PAES.
View Article and Find Full Text PDFLow Prostaglandin E but High Prostaglandin D, a Paradoxical Dissociation in Arachidonic Acid Metabolism in Aspirin-Exacerbated Airway Disease: Role of Airway Epithelium.
J Clin Med
December 2024
Institut d'Investigacions Biomèdiques August Pi I Sunyer (IDIBAPS), University of Barcelona, 08907 Barcelona, Spain.
In patients with aspirin-exacerbated respiratory disease (AERD), there is disparate regulation of prostaglandin E2 (PGE) and prostaglandin D (PGD). Both prostanoids are synthesised by cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2). However, while the basal synthesis of PGE tends to decrease, that of PGD increases in patients with AERD.
View Article and Find Full Text PDFNonsteroidal antiinflammatory drug-exacerbated respiratory disease: molecular mechanism, management and treatment.
Front Allergy
November 2024
IAPA 's Clinic, Department of Otorhinolaryngology-Head and Neck Surgery, Instituto Nacional de Enfermedades Respiratorias, Ismael Cosío Villegas, Ciudad de México, México.
It has been estimated that Nonsteroidal Anti-inflammatory drug (NSAID) Exacerbated Respiratory Disease (N-ERD) previously named as Aspirin Exacerbated Respiratory Disease (A-ERD) affects around 1.4 million persons in the United States. Its prevalence in asthmatic patients has widely been underestimated, as a considerable number of patients would need an aspirin provocation test to confirm the diagnosis.
View Article and Find Full Text PDFChronic Rhinosinusitis With Nasal Polyps Does Not Affect the Association Between the Nasal Provocation Test and Serum Allergen-Specific Immunoglobulin E Levels.
J Rhinol
March 2024
Department of Otorhinolaryngology-Head and Neck Surgery, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
Background And Objectives: This study compared nasal provocation test (NPT) results between groups with and without chronic rhinosinusitis with nasal polyps (CRSwNP) to investigate whether CRSwNP affects the response to the intranasal allergen challenge.
Methods: We reviewed the medical records of patients who had undergone the NPT, multiple allergen simultaneous test (MAST), and paranasal sinus computed tomography. Patients were diagnosed with CRSwNP based on findings from nasal endoscopy and paranasal sinus computed tomography.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!