Crystals of human T-cell leukemia virus protease (HTLV-1 PR) have been very difficult to prepare and only native data extending to 2.6 angstroms resolution could be collected. Initial attempts to solve the structure with a variety of low-sequence-identity models utilizing proteases from other retroviruses and using a number of molecular-replacement programs were unsuccessful. The structure was finally solved using Phaser, revealing extensive pseudosymmetry and significant deviations from the starting models, features that were likely to be responsible for the initial failures. The steps taken to solve this structure and some of its intriguing crystallographic aspects are discussed.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1107/S0907444905040655 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
LASP to the Future of Atomic Simulation: Intelligence and Automation.
Precis Chem
December 2024
Collaborative Innovation Center of Chemistry for Energy Material, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Key Laboratory of Computational Physical Science, Department of Chemistry, Fudan University, Shanghai 200433, China.
Atomic simulations aim to understand and predict complex physical phenomena, the success of which relies largely on the accuracy of the potential energy surface description and the efficiency to capture important rare events. LASP software (large-scale atomic simulation with a Neural Network Potential), released in 2018, incorporates the key ingredients to fulfill the ultimate goal of atomic simulations by combining advanced neural network potentials with efficient global optimization methods. This review introduces the recent development of the software along two main streams, namely, higher intelligence and more automation, to solve complex material and reaction problems.
View Article and Find Full Text PDFHydrogel microspheres for bone regeneration through regulation of the regenerative microenvironment.
Biomater Transl
September 2024
Department of Orthopaedics, Laboratory of Biological Tissue Engineering and Digital Medicine, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan Province, China.
Bone defects are a prevalent category of skeletal tissue disorders in clinical practice, with a range of pathogenic factors and frequently suboptimal clinical treatment effects. In bone regeneration of bone defects, the bone regeneration microenvironment-composed of physiological, chemical, and physical components-is the core element that dynamically coordinates to promote bone regeneration. In recent years, medical biomaterials with bioactivity and functional tunability have been widely researched upon and applied in the fields of tissue replacement/regeneration, and remodelling of organ structure and function.
View Article and Find Full Text PDFSynthesis and characterizing of MgO, 58S bioactive glass and N carboxymethyl chitosan and coating composites of them on SS316L.
Int J Biol Macromol
December 2024
Department of Materials Science and Engineering, Faculty of Engineering & Technology, Tarbiat Modares Universirty, Tehran, Iran.
One of the most effective ways to solve the problems caused by the presence of steel implants in the body is to apply a coating to them. This study aims to develop and optimize composite coatings of magnesium oxide (MgO), 58S bioactive glass (BG), and N-carboxymethyl chitosan (N-CMC) on stainless steel (SS316L) substrates using the electrophoretic deposition (EPD) method. The synthesized materials were characterized using FTIR, XRD, and SEM to confirm their structure and morphology prior to coating.
View Article and Find Full Text PDFIdentification of assembly mode of non-canonical BAF (ncBAF) chromatin remodeling complex core module.
Biochem Biophys Res Commun
December 2024
State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science, and College of Life Sciences, Nankai University, 94 Weijin Road, Tianjin, 300071, China. Electronic address:
Mammalian SWI/SNF (mSWI/SNF) ATP-dependent chromatin remodeling complexes play critical roles in regulating gene expression and DNA accessibility, and more than 20 % of cancers have mutations in genes encoding chromatin remodeling complexes. The mSWI/SNF family comprises three distinct classes: canonical BAF (cBAF), PBAF, and non-canonical BAF (ncBAF). While the structures of cBAF and PBAF have been resolved by using cryo-electron microscopy (cryo-EM), the modular organization and assembly mechanism of ncBAF remain poorly understood.
View Article and Find Full Text PDFMicroelectricity enhances aerobic granular sludge granulation and sulfamethazine degradation: Performance, mechanism, antibiotic resistance genes and microbial community.
J Environ Manage
December 2024
College of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050, PR China.
With the widespread use of typical antibiotics such as sulfamethazine (SMT), it leads to their accumulation in the environment, increasing the risk of the spread of antibiotic resistance genes (ARGs). Aerobic granular sludge (AGS) has shown great potential in treating antibiotic wastewater. However, the long cultivation period of AGS, the easy disintegration of particles and the poor stability of degradation efficiency for highly concentrated antibiotic wastewater are still urgent problems that need to be solved, and it is important to explore the migration and changes of ARGs and microbial diversity in AGS systems.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!