AI Article Synopsis
- The study investigates how a semifluorinated alkane, (perfluoro-n-hexyl)ethane (F6H2), affects the properties of egg yolk phosphatidylcholine (PC) vesicles.
- Large unilamellar vesicles (LUVs) were analyzed using techniques like fluorescence spectroscopy and zeta potential measurements, revealing that F6H2 integrates into the lipid bilayer and increases its viscosity.
- The zeta-potential becomes more negative with increasing temperature, and the presence of potassium ions (K+) enhances the stability of the liposomes according to the DLVO theory of colloidal stability.
Article Abstract
The aim of this study is to characterize vesicles obtained by the incorporation of the semifluorinated alkane, (perfluoro-n-hexyl)ethane (diblock F6H2) to a standard lipid, egg yolk phosphatidylcholine (PC). Large unilamellar vesicles (LUVs), prepared by extrusion, were characterized by fluorescence spectroscopy, zeta potential (zeta-potential) and light scattering. By using the fluorescence spectroscopy technique, the anisotropy of l,6-diphenyl-l,3,5-hexatriene (DPH) probe at different temperatures was determined. It was demonstrated that F6H2 is placed inside of the lipid bilayer and that the hydrocarbon acyl chain in the bilayers has higher viscosity in the presence of fluoroalkane. The zeta-potential of the PC-F6H2 system is negative and increases (in absolute value) from -10 to -19 mV when the temperature rises from 10 to 25 degrees C, this last value keeping practically constant with a further increase of temperature. The adsorption of K+ ions on the liposome surface was measured by zeta-potential. This adsorption originates a sudden increase of the initial zeta-potential followed by a slight decrease with K+ concentration. The application of the DLVO theory of colloidal stability showed a growing dependence of the DLVO potential with K+ concentration and consequently a increasing stability.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.colsurfb.2005.11.022 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Higher concentration of trehalose dihydrate stabilizes recombinant IgG1 under forced stress conditions.
J Pharm Sci
January 2025
Department of Chemical Engineering, Indian Institute of Technology Delhi. Electronic address:
Stability of complex biotherapeutics like monoclonal antibodies is paramount for their safe and efficacious use. Excipients are inactive ingredients that are added to the purified product so as to offer it a stable environment. Trehalose dihydrate is a non-reducing sugar that is commonly used as a stabilizing agent in biotherapeutic formulations under liquid and frozen states.
View Article and Find Full Text PDFEasy and Fast Detection of Hypochlorite by a Bithiophene-Based Fluorescent Turn-on Sensor and its Applications to Test Strips, Real Water Samples, and Smartphone-Assisted Platform.
J Fluoresc
January 2025
Department of Fine Chemistry, Seoul National University of Science and Technology, Seoul, 01811, Korea.
We report a bithiophene-based fluorescence probe BDT (2,2'-(((1 E, 1'E)-[2,2'-bithiophene]-5,5'-diylbis(methaneylylidene))bis(azaneylylidene))bis(4-(tert-butyl)phenol)) for recognizing ClO. BDT selectively responded to ClO, leading to a blue fluorescence enhancement in a mixture of DMF/HEPES buffer (9:1, v/v). Importantly, BDT showed an ultrafast response (within 1 s) to ClO among the fluorescent turn-on chemosensors based on bithiophene.
View Article and Find Full Text PDFHighly Luminescent Nitrogen Doped Carbon Quantum Dots for Mercury Ion Sensing with Antibacterial Activity.
J Fluoresc
January 2025
Department of Chemistry, The University of Burdwan, Golapbag, Burdwan, 713104, India.
Nitrogen doped Carbon Quantum Dots (NCQDs) have been synthesized using most economical and easiest hydrothermal process. Here, N-phenyl orthophenylenediamine and citric acid were utilised as a source of nitrogen and carbon for the preparation of NCQDs. The synthesized NCQDs were characterized using experimental techniques like UV - Vis absorption, FT-IR, transmission electron microscopy (TEM), X-ray Diffraction (XRD), EDX, dynamic light scattering (DLS), fluorimeter and time resolved fluorescence spectroscopy.
View Article and Find Full Text PDFEu-ion doped strontium molybdate perovskite quantum dots as a turn-off-on fluorescent sensor for simultaneous detection of hypoxanthine biomarker and Fe ions.
Mikrochim Acta
January 2025
Department of Chemistry, Sardar Vallabhbhai National Institute of Technology, Surat, Gujarat, 395 007, India.
A fluorescence "turn-off-on" nanoprobe is designed by using europium-doped strontium molybdate perovskite quantum dots (Eu:SMO PQDs) for the sequential detection of hypoxanthine (Hx) and Fe. The Eu:SMO PQDs were prepared by the sol-gel method using Sr(NO), (NH)MoO.4HO, and Eu(OCOCH) as precursors.
View Article and Find Full Text PDFGiant Purcell Broadening and Lamb Shift for DNA-Assembled Near-Infrared Quantum Emitters.
ACS Nano
January 2025
Institute of Physics, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.
Controlling the light emitted by individual molecules is instrumental to a number of advanced nanotechnologies ranging from super-resolution bioimaging and molecular sensing to quantum nanophotonics. Molecular emission can be tailored by modifying the local photonic environment, for example, by precisely placing a single molecule inside a plasmonic nanocavity with the help of DNA origami. Here, using this scalable approach, we show that commercial fluorophores may experience giant Purcell factors and Lamb shifts, reaching values on par with those recently reported in scanning tip experiments.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!