Purpose: To measure glucose flux across human, bovine, and porcine corneas and to determine the diffusion coefficient of each type of cornea.

Methods: Diffusion of glucose across human (n = 8), bovine (n = 7), and pig corneas (n = 8) was measured using a modified blind well chamber apparatus (Boyden chamber). Dialysis membranes (n = 7) and nonporous Mylar membranes (n = 7) were used as positive and negative controls, respectively. Glucose concentrations were measured at 30-min intervals with a commercially available glucose meter.

Results: The diffusion of glucose through corneas in vitro was calculated by a simple Fickian diffusion model. The diffusion coefficient of glucose is highest for the human cornea (D(HC) = 3.0 +/- 0.2 x 10(-6) cm(2)/s) followed by porcine corneas (D(PC) = 1.8 +/- 0.6 x 10(-6) cm(2)/s) and bovine corneas (D(BC) = 1.6 +/- 0.1 x 10(-6) cm(2)/s) (p < 0.05). The diffusion coefficients of all tested corneas were significantly higher (p < 0.05) than that of dialysis membrane (D(DM) = 3.4 +/- 0.2 x 10(-7) cm(2)/s).

Conclusion: The glucose diffusion coefficients of human, bovine, and porcine corneas are on the order of 10(-6). Human corneas have higher permeability to glucose than either porcine or bovine corneas.

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http://dx.doi.org/10.1159/000090726DOI Listing

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