The aim of this study was to determine the accuracy of sexing fetal cells isolated from cervical mucus. Immunopositive cells identified using a fetal-specific antibody were isolated and subjected to genetic analysis. Fetal sex was predicted in all 22 samples, as confirmed by analysis of the corresponding placental tissue. This study validates the accuracy of our test for sex diagnosis and confirms the ability to effectively recover and analyse fetal cells from the cervical mucus during early pregnancy.
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http://dx.doi.org/10.1111/j.1479-828X.2005.00492.x | DOI Listing |
J Cell Mol Med
March 2025
Center for Reproductive Medicine, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, People's Republic of China.
Joubert syndrome (JS) is a rare neurodevelopmental disorder associated with mutations in genes involved in ciliary function. Germline variants in CPLANE1 have been implicated in JS. In this study, we investigated a family with three adverse pregnancies characterised by fetal malformations consistent with JS.
View Article and Find Full Text PDFInvest Ophthalmol Vis Sci
March 2025
Department of Bioengineering, University of Texas at Dallas, Richardson, Texas, United States.
Purpose: After stromal injury to the cornea, the release of growth factors and pro-inflammatory cytokines promotes the activation of quiescent keratocytes into a migratory fibroblast and/or fibrotic myofibroblast phenotype. Persistence of the myofibroblast phenotype can lead to corneal fibrosis and scarring, which are leading causes of blindness worldwide. This study aims to establish comprehensive transcriptional profiles for cultured corneal keratocytes, fibroblasts, and myofibroblasts to gain insights into the mechanisms through which these phenotypic changes occur.
View Article and Find Full Text PDFCells
February 2025
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia.
Although every cell biologist knows the importance of selecting the right growth conditions and it is well known that the composition of growth medium may vary depending on a product brand or lot affecting many cellular processes, still those effects are poorly systematized. We addressed this issue by comparing the effect of 12 fetal bovine sera (FBS) and eight growth media from different brands on the morphological and functional parameters of five cell types: lung adenocarcinoma, neuroblastoma, glioblastoma, embryonic kidney, and colorectal cancer cells. Using high-throughput imaging, we compared cell proliferation; performed morphological profiling based on the imaging of 561,519 cells; measured extracellular regulated kinases (ERK1/2) activity, mitochondria potential, and lysosome accumulation; and compared cell sensitivity to drugs, response to EGF stimulation, and ability to differentiate.
View Article and Find Full Text PDFMater Today Bio
April 2025
Institute of Metal Research, Chinese Academy of Sciences, Shenyang, Liaoning 110016, China.
The underlying mechanisms governing the interactions between nanoparticles and vascular endothelial barrier remain largely unexplored, which is crucial for the optimal design of nanoparticles for clinical applications. In this study, the size-dependent interactions between calciprotein particles (CPPs) and endothelial cells (ECs) were investigated using a rat model of chronic kidney disease (CKD) induced by 5/6 nephrectomy. Two primary types of CPP1 were studied: small-sized CPP1 (S-CPP1, <50 nm) and larger CPP1 (L-CPP1, <100 nm), detected three and five weeks post-surgery, respectively.
View Article and Find Full Text PDFJ Appl Toxicol
March 2025
Department of Environment and Public Health, National Institute for Minamata Disease, Minamata, Kumamoto, Japan.
Despite the high frequency of pregnancies complicated by abnormal glucose metabolism associated with obesity, methylmercury (MeHg) metabolism in pregnant women with abnormal glucose metabolism is unclear. We aimed to elucidate the MeHg tissue distribution in obese female mice with abnormal glucose metabolism and their fetuses. Female C57BL/6J mice were fed a high-fat diet (HFD) or a standard diet (Ctrl) for 12 weeks and mated.
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